Liquid Chromatography

Would you like to learn some useful tips for preventive maintenance of your LC system and know what to look out for to keep it running at optimal performance? If so, this upcoming webinar from Separation Science, in collaboration with Agilent, will...

This application note provides details of LC conditions and columns to achieve high resolution and high-speed analysis of UV-absorbents using an SPP column.

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

Shimadzu has announced the release of its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). It provides better prep processes for extraction of target compounds and impurities during drug discovery in the pharmaceutical...

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

Covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation. You should take this course if you are new to HPLC, or do you supervise new users of HPLC.

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such demanding...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

This article discusses the pH of the mobile phase that should be selected in order to enhance the hydrophilicity of the analytes.

In this technical article the effect of the salt content in HILIC mobile phases and the preparation is discussed.

In HILIC, small variations of the elution conditions often result in differences in the retention greater than the ones observed in RPLC. Therefore, particular attention has to be paid to the selection and the preparation of the eluents. 

A common and useful classification of the HILIC stationary phases is on the basis of the presence of functional groups on the surface and their charged state: they can be divided in unbounded and bonded phases, and these ones are classified in...

In this article, we consider whether it is ok to back-flush a column. If a C18 column is used for a routine analysis and the pressure has risen, as in this example, if the column is reversed and unplugged will this be ok or will be the column be...