Liquid Chromatography Blog

How to make the most of your LC system

Would you like to learn some useful tips for preventive maintenance of your LC system and know what to look out for to keep it running at optimal performance? If so, this upcoming webinar from Separation Science, in collaboration with Agilent,...

Pore Size vs Particle Size

In this technical article you'll learn about the roles of pore size and particle size for an HPLC packing material.

Does a Guard Column Make Sense?

This article explains how a guard column is one of those HPLC accessories that is a bit of a mixed grill – it can be a good thing or a not-so-good thing. Different aspects of guard column use will be assessed to see if they make sense for you.

What's New at the Largest Liquid-Phase Separations Conference in the World

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

New Multi-Channel UV Detection Systems for Preparative and Flash Chromatography

Learn more about solid state detectors for Preparative Chromatography, which offer higher sensitivity through a 220 nm laser channel for narrow-band precision and as a result is 100 times more stable than Deuterium Lamps.

The Basics of HILIC

How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.

Do you HILIC?

Are your analytes eluting close to the void volume of a reversed-phase (RP) column? Do you have problems retaining organic molecules under conventional reversed‑phase liquid chromatography (RPLC) conditions? Have you increased the initial...

Chromatography software to support more HPLC systems

DataApex is pleased to announce that Clarity VA Chromatography Software supporting the Hitachi Chromaster and Primaide HPLC systems is now available in the North American market. This special Clarity version is exclusively available through...

Acid in A and B

I had a follow-up enquiry about one of the recent articles. In 'Why Acid?', I discussed some of the reasons why acids such as formic or trifluoroacetic (TFA) are added to mobile phases for LC-MS work. The reader noted that in Figure 1 of that...

Injection Problems

Q: I am running an isocratic method with a 250 x 4.6 mm, 10 µm particle column at 1 mL/min with a 20% acetonitrile (ACN)/buffer mobile phase. I dissolve my sample in ACN and inject. Normally my compound of interest comes out at 7.7 min. I...

Frit Cleaning

This technical article looks at the best way to clean the frits used in the HPLC system. In this example, the reader had been sonicating them in nitric acid, but wasn’t sure if that was the best way to approach the problem.

Retention Shifts

A reader complained about retention times that changed in an overnight HPLC run and wondered how to diagnose the problem source. The question left too many possibilities, and because there was no complaint about changing resolution, I’ll assume...

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