Liquid Chromatography

Having trouble separating compounds, and wondering what is the single most important parameter to change? Questions over characterizing high molecular weight compounds such as oligonucleotides? Tackle column selection like an expert by registering...

Separation Science, in collaboration with Waters, has developed a series of open-access online LC learning courses covering choosing base particles, ligands and hardware for robust chromatographic separations. We are pleased to offer a new full...

Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices.

LC-MS systems are complex and sensitive instruments, and even minor issues can lead to inaccurate results or instrument downtime, resulting in financial losses and delayed research.

This article from Issue 14 of the Analytix Reporter discusses theHPTLC system suitability test (SST) which often qualifies only a limited region of the chromatogram. The newly developed Universal HPTLC Mix (UHM) enables TLC users to efficiently and...

This article from Issue 14 of the Analytix Reporter discusses thin-layer chromatography (TLC), a fast, easy-to-use, cost-efficient, and highly versatile separation technique for qualitative and quantitative analysis. It describes some of the key...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we review some basic chromatographic measurements – N, α, k, As, TF, Rs – and how these play into a method development strategy...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We concentrate on reversed-phase separations in this course, because the majority of HPLC and UHPLC methods use this technique. How does...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. How can we take advantage of pH to improve the separation and what are the pitfalls when we do use pH for this purpose? Why is...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the...