Liquid Chromatography

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we review some basic chromatographic measurements – N, α, k, As, TF, Rs – and how these play into a method development strategy...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We concentrate on reversed-phase separations in this course, because the majority of HPLC and UHPLC methods use this technique. How does...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. How can we take advantage of pH to improve the separation and what are the pitfalls when we do use pH for this purpose? Why is...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of...

Do you work with HPLC or UHPLC? "HPLC & UHPLC Troubleshooting: A Performance Qualification Approach” is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography...

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...