Liquid Chromatography

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and...

Join scientists from around the world at the 50th sequel of the longest-running, largest, and most recognized conference in the world covering all aspects of Separations and Analyses carried out in Liquid Phase. HPLC 2022 will take place on June...

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.

Are your analytes eluting close to the void volume of a reversed-phase (RP) column? Do you have problems retaining organic molecules under conventional reversed‑phase liquid chromatography (RPLC) conditions? Have you increased the initial percentage...

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such demanding...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

This article discusses the pH of the mobile phase that should be selected in order to enhance the hydrophilicity of the analytes.

In this technical article the effect of the salt content in HILIC mobile phases and the preparation is discussed.

In HILIC, small variations of the elution conditions often result in differences in the retention greater than the ones observed in RPLC. Therefore, particular attention has to be paid to the selection and the preparation of the eluents. 

A common and useful classification of the HILIC stationary phases is on the basis of the presence of functional groups on the surface and their charged state: they can be divided in unbounded and bonded phases, and these ones are classified in...

This video provides a general overview of hydrophilic interaction chromatography, also known as HILIC.HILIC separation is based on hydrophilic interaction. HILIC uses the same solvent as with reserved-phase chromatography (acetonitrile / water /...

Shodex's new range of columns are available for a wide range of separation techniques. The separation techniques start with reversed phase, normal phase, HILIC, ion chromatography, ion exchange, ion exclusion, size-exclusion chromatography,...