Liquid Chromatography

Analysis of size distribution of a protein hydrolysate with Superdex™ 30 Increase size exclusion chromatography column

There is a growing interest in protein hydrolysates. It has been shown that short bioactive peptides can have higher nutritive value, for example, as antioxidants. In the characterization of protein hydrolysates, it is important to analyse the...

Use of Superdex™ 200 Increase size exclusion chromatography columns for screening of mAb aggregates

Screening for mAb aggregation is important in the production of mAb. When performing aggregate analysis on mAbs there is often no issue when the aggregate level is above 1%. At lower levels, however, issues can arise in integration of the...

New HPLC columns from Shodex to improve your separation performance

Shodex's new range of columns are available for a wide range of separation techniques. The separation techniques start with reversed phase, normal phase, HILIC, ion chromatography, ion exchange, ion exclusion, size-exclusion chromatography,...

Understanding GPC/SEC - Finding the Right Particle Size and Pore Size

Want to understand what the correct criteria for column selection should be based on? If so, this tutorial is for you...

Understanding GPC/SEC - Unexpected Peak Shapes

Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts?

Understanding GPC/SEC - How to deal with pressure problems

So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

Understanding GPC/SEC - What do you need to know before injecting a sample?

So you're thinking of using gel permeation chromatography (GPC) for your analyses, but before you dive in just what information do you need to know before your first sample injection?

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