Liquid Chromatography Blog

Separation Science offers a series of learning modules covering basics, best practice and troubleshooting in GPC/SEC. This multipart series covers such topics as:

Want to get a better understanding of the various sample preparation procedures available for GPC/SEC analysis, and what to consider when selecting one? If so, this tutorial is for you...

There is a growing interest in protein hydrolysates. It has been shown that short bioactive peptides can have higher nutritive value, for example, as antioxidants. In the characterization of protein hydrolysates, it is important to analyse the...

Screening for mAb aggregation is important in the production of mAb. When performing aggregate analysis on mAbs there is often no issue when the aggregate level is above 1%. At lower levels, however, issues can arise in integration of the...

Shodex's new range of columns are available for a wide range of separation techniques. The separation techniques start with reversed phase, normal phase, HILIC, ion chromatography, ion exchange, ion exclusion, size-exclusion chromatography,...

Malvern has produced an application note describing the GPC (or equivalently SEC) analysis of branching in polyvinylpyrrolidone (PVP).

Malvern has produced an application note describing molecular and rheological changes that occurred as samples of PS and PMMA were extruded through a capillary rheometer, simulating moulding.

Want to understand what the correct criteria for column selection should be based on? If so, this tutorial is for you...

Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts?

So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

Tosoh Bioscience has produced an application note describing how polyphenylene sulfide (PPS) analysis can be successfully achieved using its EcoSEC high temperature GPC system.

Tosoh Bioscience has produced an application note describing the analysis of asphalt binders using gel permeation chromatography.