Liquid Chromatography

Calibration in GPC/SEC

Separation Science offers a series of learning modules covering basics, best practice and troubleshooting in GPC/SEC. This multipart series covers such topics as:

Sample Preparation in GPC/SEC

Want to get a better understanding of the various sample preparation procedures available for GPC/SEC analysis, and what to consider when selecting one? If so, this tutorial is for you...

Analysis of size distribution of a protein hydrolysate with Superdex™ 30 Increase size exclusion chromatography column

There is a growing interest in protein hydrolysates. It has been shown that short bioactive peptides can have higher nutritive value, for example, as antioxidants. In the characterization of protein hydrolysates, it is important to analyse the...

Use of Superdex™ 200 Increase size exclusion chromatography columns for screening of mAb aggregates

Screening for mAb aggregation is important in the production of mAb. When performing aggregate analysis on mAbs there is often no issue when the aggregate level is above 1%. At lower levels, however, issues can arise in integration of the...

New HPLC columns from Shodex to improve your separation performance

Shodex's new range of columns are available for a wide range of separation techniques. The separation techniques start with reversed phase, normal phase, HILIC, ion chromatography, ion exchange, ion exclusion, size-exclusion chromatography,...

GPC/SEC Analysis of Polymer Branching

Malvern has produced an application note describing the GPC (or equivalently SEC) analysis of branching in polyvinylpyrrolidone (PVP).

Characterizing Different Polymer Degradation Routes during Processing using Multi-detector GPC and Capillary Rheology

Malvern has produced an application note describing molecular and rheological changes that occurred as samples of PS and PMMA were extruded through a capillary rheometer, simulating moulding.

Understanding GPC/SEC - Finding the Right Particle Size and Pore Size

Want to understand what the correct criteria for column selection should be based on? If so, this tutorial is for you...

Understanding GPC/SEC - Unexpected Peak Shapes

Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts?

Understanding GPC/SEC - How to deal with pressure problems

So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

How to Achieve High Temperature GPC Analysis of Polyphenylene Sulfide

Tosoh Bioscience has produced an application note describing how polyphenylene sulfide (PPS) analysis can be successfully achieved using its EcoSEC high temperature GPC system.

Effective Analysis of Asphalt Binders

Tosoh Bioscience has produced an application note describing the analysis of asphalt binders using gel permeation chromatography.

Analysis of Bio-Inspired Alkylalanoid Polymers

Tosoh Bioscience has produced an application describing how to improve reproducibility of MW measurements of polypeptoids using an EcoSEC GPC system.

Monitoring Single-Chain Nanoparticle Formation

Tosoh Bioscience has produced an application note describing how to monitor single-chain nanoparticle formation and characterize nanoparticles using multi-pore TSKgel GPC columns.

High Temperature GPC Analysis of a Polypropylene Copolymer

Tosoh Bioscience has produced an application note determining the molar mass averages and polydispersity of two polypropylene random copolymer samples via refractive index (RI) detection using the EcoSEC High Temperature GPC System and TSKgel...

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