This article discusses the pH of the mobile phase that should be selected in order to enhance the hydrophilicity of the analytes.
All the analytical scientists that work with reversed-phased liquid chromatography (RPLC) know really well that the pH of the mobile phase determines the ionization state of the analytes. That remains valid also in HILIC, but differently from RPLC, the pH of the HILIC mobile phase should be selected with the intention of bringing the analytes in their ionic form. Indeed, the charged form of a compound is in general more hydrophilic in comparison with its neutral form, and, therefore, it will be more retained in HILIC conditions.
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