Liquid Chromatography Blog

Shimadzu has announced the release of its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). It provides better prep processes for extraction of target compounds and impurities during drug discovery in the pharmaceutical...

Learn how complementary TLC-MS and TLC-HPLC-MS methods can be used for successful analysis of neonicotinoid pesticides in honey.

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

Sample preparation is an integral part to successful chromatographic analysis. Selecting the right sample preparation technique is dependent upon...

Merck has introduced our new proficiency testing data entry portal. The new portal, released 2018, can be accessed at Merck-pt.com. The portal has been streamlined to offer speed, ease of use, and data handling. 

How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.

Are your analytes eluting close to the void volume of a reversed-phase (RP) column? Do you have problems retaining organic molecules under conventional reversed‑phase liquid chromatography (RPLC) conditions? Have you increased the initial...

DataApex is pleased to announce that Clarity VA Chromatography Software supporting the Hitachi Chromaster and Primaide HPLC systems is now available in the North American market. This special Clarity version is exclusively available through...

I had a follow-up enquiry about one of the recent articles. In 'Why Acid?', I discussed some of the reasons why acids such as formic or trifluoroacetic (TFA) are added to mobile phases for LC-MS work. The reader noted that in Figure 1 of that...

Q: I am running an isocratic method with a 250 x 4.6 mm, 10 µm particle column at 1 mL/min with a 20% acetonitrile (ACN)/buffer mobile phase. I dissolve my sample in ACN and inject. Normally my compound of interest comes out at 7.7 min. I...

In a recent email, a reader enquired about the best way to clean the frits used in the HPLC system. He indicated that he had been sonicating them in nitric acid, but wasn’t sure if that was the best way to approach the problem.

A reader complained about retention times that changed in an overnight HPLC run and wondered how to diagnose the problem source. The question left too many possibilities, and because there was no complaint about changing resolution, I’ll assume...