Liquid Chromatography Blog

Pittcon has been a face-to-face conference and exposition for 71 years, and it is their 72nd installment that they have decided to boldly embrace this ever-changing age. This March 8 – 12 will mark an historic transition reflective of the times:...

Interferences exist in every chromatographic analysis whether they are recognized or not. Interferences can be visible in your chromatogram and present themselves as reduced peak area counts, split peaks, shoulders around the peak, or peak...

A successful chromatographic analysis starts with the sample. Sample selection, collection, transportation, preservation, storage, and sampling protocol are all aspects of testing that need to be considered in the context of your analytical...

While food and beverage imports and exports continue to increase, regulating bodies are taking a greater interest in these activities that can affect public health. Since the early 2000s, worldwide food imports have increased dramatically, as...

In the last column, we discussed ‘just enough’ sample preparation techniques, such as filtration, protein precipitation, extraction partitioning, solid-supported liquid extraction and QuEChERS. Now we will discuss when ‘just enough’ is not enough...

We’ve covered many of the types of sample preparation options available to make samples ready for instrumental analysis by gas or liquid chromatography and mass spectrometry detection. There are techniques such as QuEChERS, liquid-liquid...

A reader wrote me recently asking what would happen if he injected his sample, which came dissolved in hexane, into a reversed-phase mobile phase of methanol-buffer. The first answer is that this is not a good habit, but is it possible? We’ll see.

I recently had an inquiry from a reader of 'HPLC Solutions' that comes up with some regularity in the HPLC troubleshooting classes that I teach: “Will I damage the column by getting air in it?” Let’s consider the possibilities for a moment. One...

A reader asked me if she was in danger of shortening the lifetime of an HPLC column by removing it after each batch of samples and storing it. This also raised the related question about expectations of shorter column lifetimes if the same...

What happens if your favourite HPLC column accidentally rolls off the bench and lands on the concrete floor of the lab? Is it ruined?

After I wrote an article on in-line Degasser (HPLC solutions #72), I immediately received some reader feedback on a specific failure, which was published in 'Failed Degasser' (HPLC solutions #73). Since that time I have been collecting occasional...

A reader reported a problem blockage of a column packed with one of the newer shell-type packings. The mobile phase A comprises a mixture of 0.1 M KH₂PO₄, 0.05% triethylamine (TEA), and 150 mg/L EDTA adjusted to pH 7.5 with NaOH. The B-solvent is...