Liquid Chromatography

The sensitivity of a chromatographic method might be described by its limit of detection (LOD). LOD is usually defined as the minimum detectable amount or concentration of a component that can be reliably detected using a given analytical method....

The mobile phase in HPLC strongly influences the chromatographic separation. Its preparation can be a source of trouble for a method, if not done correctly and consistently. This article provides some suggestions and practical tips for the mobile...

In this article the selection of appropriate robustness factors and factor levels relating to the mobile phase will be discussed and in particular, the volume fraction of the organic solvent in the mobile phase for reversed phase HPLC.

Shimadzu has announced the release of its “Advanced i-Series” liquid chromatographs meeting user demands for analysing a large number of samples or quickly re-processing data even when working from home.

In this on-demand webinar, the advantages of the ACQUITY PREMIER columns are highlighted and the various particle technologies and their benefits in chromatographic separations discussed. Register for the on-demand webinar and see what you've...

Would you like to learn some useful tips for preventive maintenance of your LC system and know what to look out for to keep it running at optimal performance? If so, this upcoming webinar from Separation Science, in collaboration with Agilent,...

This application note provides details of LC conditions and columns to achieve high resolution and high-speed analysis of UV-absorbents using an SPP column.

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

Shimadzu has announced the release of its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). It provides better prep processes for extraction of target compounds and impurities during drug discovery in the pharmaceutical...

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

Covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation. You should take this course if you are new to HPLC, or do you supervise new users of HPLC.

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.