Liquid Chromatography Blog

Particle technologies and their benefits in chromatographic separations

In this upcoming webinar, the advantages of the ACQUITY PREMIER columns will be highlighted and the various particle technologies and their benefits in chromatographic separations will be discussed. Register now and see what you've been missing!

How to make the most of your LC system

Would you like to learn some useful tips for preventive maintenance of your LC system and know what to look out for to keep it running at optimal performance? If so, this upcoming webinar from Separation Science, in collaboration with Agilent,...

High-resolution and high-speed simultaneous analysis of regulated UV-adsorbents in cosmetics

This application note provides details of LC conditions and columns to achieve high resolution and high-speed analysis of UV-absorbents using an SPP column.

What's New at the Largest Liquid-Phase Separations Conference in the World

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

Better prep for multi-sampling processing

Shimadzu has announced the release of its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). It provides better prep processes for extraction of target compounds and impurities during drug discovery in the pharmaceutical...

Groundbreaking LC technology to merge ease-of-use and peace-of-mind

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

HILIC & MS

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such...

Sample Solvent in HILIC

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

Column Equilibration in HILIC

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Column temperature in HILIC

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

HILIC Mobile Phase: pH

The pH of the mobile phase should be selected in order to enhance the hydrophilicity of the analytes.

HILIC Mobile Phase: Salt Content

The addition of salt to HILIC mobile phases is strongly recommended to improve reproducibility and peak shape. Do you know how to solubilize the salt in mobile phases at high acetonitrile content?

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