Liquid Chromatography Blog

Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible...

Shimadzu has announced the release of its new Nexera Prep Series Preparative Purification Liquid Chromatograph (LC). It provides better prep processes for extraction of target compounds and impurities during drug discovery in the pharmaceutical...

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

The pH of the mobile phase should be selected in order to enhance the hydrophilicity of the analytes.

The addition of salt to HILIC mobile phases is strongly recommended to improve reproducibility and peak shape. Do you know how to solubilize the salt in mobile phases at high acetonitrile content?

In HILIC, small variations of the elution conditions often result in differences in the retention greater than the ones observed in RPLC. Therefore, particular attention has to be paid to the selection and the preparation of the eluents. 

A common and useful classification of the HILIC stationary phases is on the basis of the presence of functional groups on the surface and their charged state: they can be divided in unbounded and bonded phases, and these ones are classified in...