Liquid Chromatography

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

The pH of the mobile phase should be selected in order to enhance the hydrophilicity of the analytes.

The addition of salt to HILIC mobile phases is strongly recommended to improve reproducibility and peak shape. Do you know how to solubilize the salt in mobile phases at high acetonitrile content?

In HILIC, small variations of the elution conditions often result in differences in the retention greater than the ones observed in RPLC. Therefore, particular attention has to be paid to the selection and the preparation of the eluents. 

A common and useful classification of the HILIC stationary phases is on the basis of the presence of functional groups on the surface and their charged state: they can be divided in unbounded and bonded phases, and these ones are classified in...

DataApex has produced a white paper providing application examples of its Clarity software for analytical and small-scale preparative separation using multivendor analytical and preparative HPLC components.

Thermo Fisher Scientific offers a number of resources to help you learn how charged aerosol detection can be used for both routine and complex analyses and understand more about the versatility of this novel technique.

Looking for an LC system that can improve your analytical, instrumental, and laboratory efficiency? If so, find out more about the Agilent 1260 Infinity II Prime LC...