Liquid Chromatography

How much sample can you inject into your HPLC system or chosen method? What are the limitations and boundaries? Read more in this edition of 'Tips & Tricks' in Issue 13 of the Analytix Reporter for an overview and advice on injection volumes in HPLC.

Discover the features, advantages and benefits of synthetic carbon separation media in both liquid and gas purifications and how they bring value to a wide range of LPLC applications for both small and large molecule separations by attending this...

These latest application notes cover a wide range of subjects for HPLC analysis such as separation of water-soluble vitamins, high-throughput bottom-up proteome profiling, mRNA impurity determination, high-throughput analysis of oligonucleotides and...

Some samples do not easily release water for Karl Fischer determinations. So, what are the workable options in such instances? This article from Issue 12 of the Analytix Reporter, produced by Merck, discusses the steps that can be taken to release...

Size-Exclusion Chromatography (SEC) is a powerful technique for protein characterization that requires an understanding of the principles to be successful. In this open-access course, we will discuss the history and science of SEC, its strengths and...

Successful chromatographic separations are as much about preventing the interactions you don’t want, as it is about creating the interactions you need. This open-access course is designed to take you from beginner to advanced in controlling the...

Have you ever wondered how the LC column creates a separation? What attributes are important, what do they do, and how to use them to your advantage? This open-access course is designed to take you from beginner to advanced in understanding column...

Join scientists from around the world at the 50th sequel of the longest-running, largest, and most recognized conference in the world covering all aspects of Separations and Analyses carried out in Liquid Phase. HPLC 2022 will take place on June...

This eBook demonstrates the six coveted advantages you gain with the Agilent 6546 LC/Q-TOF.

The mobile phase in HPLC strongly influences chromatographic separations. Its preparation can prove to be a source of trouble for a method, if not done correctly and consistently. Part two of this series on mobile phase preparation focuses on...

In this article from Issue 11 of the Analytix Reporter, produced by Merck, learn about the Supelco^® HPLC columns portfolio and the best practices for connecting the column to any instrument to get maximum performance.

This article from Issue 11 of the Analytix Reporter, produced by Merck, describes the addition of two new certified solution mixtures of labeled amino acids to the company's portfolio. These certified reference material (CRM) mixes are suitable for...

The sensitivity of a chromatographic method might be described by its limit of detection (LOD). LOD is usually defined as the minimum detectable amount or concentration of a component that can be reliably detected using a given analytical method....

The mobile phase in HPLC strongly influences the chromatographic separation. Its preparation can be a source of trouble for a method, if not done correctly and consistently. This article provides some suggestions and practical tips for the mobile...

In this article the selection of appropriate robustness factors and factor levels relating to the mobile phase will be discussed and in particular, the volume fraction of the organic solvent in the mobile phase for reversed phase HPLC.