Pharmaceutical Analysis

This application note describes a method for the analysis of Class 2 solvents (water-soluble samples) that are unsuited to Headspace GC methods.

This application note describes the high-speed analysis of linezolid following the draft guidance of International Harmonization of Pharmacopoeias.

This application note will help you readily perform comprehensive targeted analysis of cell culture media and understand how to identify and quantify unknown cell culture media components with high accuracy using Q-TOF MS/MS.

This application note demonstrates how the combination of UV and a single quadrupole mass detector is a powerful tool for (active pharmaceutical ingredient) API analysis in early stages of development, enabling confirmation of expected impurities.

This application note will help you understand how trap-free 2D LC-MS allows online conversion of non-volatile to volatile mobile phase conditions and how the LCMS-9030’s high mass accuracy can be used to conduct structural analysis of impurities.

This article discusses the most popular detection principles in use in industry and CE-related good working practice.

A short course in the systematic validation of HPLC methods. This section covers various aspects of data analysis including how and when external standardization, internal standardization, and the method of standard additions should be used for...

Tosoh Bioscience has produced Volume 1 of its 'Bioprocessing Application Notebook' that comprises over twenty application notes covering important aspects of biopharmaceutical purification.

We discussed the significance of a constant electro-osmotic flow and good capillary conditioning for precision in our CE methods. Now we look at the importance of injection settings for precision. We will also have a first look at sample stacking...

A short course in the systematic validation of HPLC methods. We introduce Quality by Design (QbD) as a laboratory lifestyle described in ICH Q8. QbD translates into more efficient method development, easier validation, more manageable methods in...

OPEN-ACCESS HPLC TRAINING TUTORIALS FROM KEY EXPERTS 

Although “small molecule” and “biomolecular” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with both....

Are you looking for solutions to your biopharmaceutical workflows? Struggling with your intact protein analysis, or need help with glycosylation studies? If so Separation Science, in collaboration with Thermo Fisher Scientific, has developed an...

In previous issues, we saw the importance of a controlled, constant electro-osmotic flow (EOF) for proper precision. In this issue, we will therefore look into the different strategies for capillary conditioning, as a good conditioning procedure is...

In recent years, quantitative NMR (qNMR) has become widely accepted as a very efficient and precise method for the quantification of organic compounds and is increasingly used in the pharmaceutical and chemical industry. Its main advantage, when...

This application from Agilent describes the 2D-LC analysis of complex peptones. Active solvent modulation proved to be valuable to maintain chromatographic performance and avoid breakthrough in the second dimension.