Pharmaceutical Analysis Blog

OPEN-ACCESS HPLC TRAINING TUTORIALS FROM KEY EXPERTS 

Although “small molecule” and “biomolecular” separations have traditionally been considered as separate activities, analysts in the biopharmaceutical industry regularly have to deal with...

Are you looking for solutions to your biopharmaceutical workflows? Struggling with your intact protein analysis, or need help with glycosylation studies? If so Separation Science, in collaboration with Thermo Fisher Scientific, has developed an...

In previous issues we saw the importance of a controlled, constant electro-osmotic flow (EOF) for proper precision. In this issue we will therefore look into the different strategies for capillary conditioning, as a good conditioning procedure is...

In recent years, quantitative NMR (qNMR) has become widely accepted as a very efficient and precise method for the quantification of organic compounds and is increasingly used in the pharmaceutical and chemical industry. Its main advantage, when...

This application from Agilent describes the 2D-LC analysis of complex peptones. Active solvent modulation proved to be valuable to maintain chromatographic performance and avoid breakthrough in the second dimension.

A short course in the systematic validation of HPLC methods. We go through the various validation steps in detail. We review the key regulatory requirements from the International Committee on Harmonization (ICH), which also apply to the national...

Recently, the evolution of middle to large biomacromolecules such as peptides or proteins is apparent in the market of pharmaceutical drugs in addition to traditional active ingredients based on small molecules. The use of peptides for clinical...

The aim of this study was to develop a rapid, cost-effective, modified USP <467> HS-GC-FID method for residual solvent determination in pharmaceutical products using the Thermo Scientific™ TriPlus™ 500 Headspace Autosampler and nitrogen as...

This application note provides an evaluation of the quantitative performance of the Thermo Scientific™ TriPlus™ 500 Gas Chromatography Headspace Autosampler for determination of genotoxic nitrosamines in Valsartan according to the Chinese...

Phosphorylated compounds such as nucleotides are known to interact with stainless steel components leading to recovery issues in HPLC and LC/MS. Instrument and column inertness ensure successful analysis. Discover more about the bio-inert...

Benefit from a series of educational resources aimed at pharmaceutical scientists, ranging from pharmaceutical impurity method development, to a complete HPLC workflow comprising multiple detectors, to strategies to improve productivity and...

The small diameter of the capillary in capillary electrophoresis makes for a very good heat dissipation compared with conventional electrophoresis. As a consequence, much higher voltages can be applied before heat dissipation becomes an issue.