Liquid Chromatography

PEEK (poly-ether-ether-ketone) tubing and fittings have become a standard part of most HPLC systems today. The flexibility and ease of cutting the tubing, coupled with the convenience of finger-tightened fittings make a combination that is hard to...

This article considers the dilemma that occurs with any discussion regarding method alteration in that it can be open to interpretation.

This technical article discusses the possible effect caused from interactions between changes from different variables.

The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such demanding...

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

This article discusses the pH of the mobile phase that should be selected in order to enhance the hydrophilicity of the analytes.

In this technical article the effect of the salt content in HILIC mobile phases and the preparation is discussed.

In HILIC, small variations of the elution conditions often result in differences in the retention greater than the ones observed in RPLC. Therefore, particular attention has to be paid to the selection and the preparation of the eluents. 

A common and useful classification of the HILIC stationary phases is on the basis of the presence of functional groups on the surface and their charged state: they can be divided in unbounded and bonded phases, and these ones are classified in...

Do you want to know how sample preparation for potentiometric and Karl Fischer titration can be made safer, more efficient and more reproducible? If so, sign up for this educational presentation.

This technical article discusses the injection volume, column temperature and detector wavelength adjustments.

In this article we’ll look at flow-rate and particle-size adjustments.

In this article, we’ll take a closer look at some of the potential adjustments related to the column.