This technical article discusses the injection volume, column temperature and detector wavelength adjustments.
In the last few articles we've focused on the guidelines for method adjustment listed in the European Pharmacopoeia (EP) and United States Pharmacopoeia (USP). We looked at pH (Part 1), mobile-phase composition (Part 2), column dimensions (Part 3), and flow rate and particle size (Part 4). This week we’ll focus on the remaining variables listed in Table 1.
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