Liquid Chromatography

Learn how complementary TLC-MS and TLC-HPLC-MS methods can be used for successful analysis of neonicotinoid pesticides in honey.

Shimadzu's latest issue covers selected analytical applications as well as the latest product information. From groundbreaking LC technology, trace detection of sulfur compounds with SCD and a 20 year anniversary, there is a wealth of information...

This technical article shows how sample preparation is an integral part to successful chromatographic analysis and how selecting the right sample preparation technique is dependent upon a number of factors.

Covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation. You should take this course if you are new to HPLC, or do you supervise new users of HPLC.

Merck has introduced our new proficiency testing data entry portal. The new portal, released 2018, can be accessed at Merck-pt.com. The portal has been streamlined to offer speed, ease of use, and data handling. 

How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.

Are your analytes eluting close to the void volume of a reversed-phase (RP) column? Do you have problems retaining organic molecules under conventional reversed‑phase liquid chromatography (RPLC) conditions? Have you increased the initial percentage...

DataApex is pleased to announce that Clarity VA Chromatography Software supporting the Hitachi Chromaster and Primaide HPLC systems is now available in the North American market. This special Clarity version is exclusively available through Hitachi...

I had a follow-up enquiry about one of the recent articles. In 'Why Acid?', I discussed some of the reasons why acids such as formic or trifluoroacetic (TFA) are added to mobile phases for LC-MS work. The reader noted that in Figure 1 of that...

Q: I am running an isocratic method with a 250 x 4.6 mm, 10 µm particle column at 1 mL/min with a 20% acetonitrile (ACN)/buffer mobile phase. I dissolve my sample in ACN and inject. Normally my compound of interest comes out at 7.7 min. I switched...

This technical article looks at the best way to clean the frits used in the HPLC system. In this example, the reader had been sonicating them in nitric acid, but wasn’t sure if that was the best way to approach the problem.

A reader complained about retention times that changed in an overnight HPLC run and wondered how to diagnose the problem source. The question left too many possibilities, and because there was no complaint about changing resolution, I’ll assume that...

Recently, a reader sent a question about how to get started with a method that required separation of enantiomers by reversed-phase HPLC. Unfortunately, the problem is a non-starter in this context. Enantiomers cannot be separated by reversed-phase...

A reader recently sent in this e-mail: “I am trying to find information that I can put in an SOP regarding how long a mobile phase can be used before it must be discarded. I’ve heard 1-2 weeks from some sources, whereas others say a month, and some...

This technical article addresses why formic acid would be specified as an additive for the mobile phase in an HPLC method. Formic or trifluoroacetic acid at 0.1% concentrations are common, especially for LC-MS work. There are a number of reasons for...