Liquid Chromatography

Water content determination is a standard application in many samples and Karl Fischer titration is the most widely used and accurate method. Metrohm offers solutions enabling the water content determination in almost any matrix ranging from solid...

This video describes the separation mode of ligand exchange (LEX). The main application is the complex analysis of sugars. The LEX columns always have a double separation mode. This means two different separation modes in one column. Ligand exchange...

This video provides a general overview of hydrophilic interaction chromatography, also known as HILIC.HILIC separation is based on hydrophilic interaction. HILIC uses the same solvent as with reserved-phase chromatography (acetonitrile / water /...

In this video one of the most used separation modes -  the reversed phase is being described.

Preparative-scale chromatography plays a critical role in applications where compounds must be synthesized, identified, isolated, purified, characterized, screened, and tested. Waters AutoPurification Systems offer scalable prep configurations,...

The transfer of chromatographic methods occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one laboratory to another within the same organization, holding all instrumental and...

The Ultivo from Agilent Technologies is a small triple quadrupole LC/MS system designed to maximize quantitative performance and promote more uptime. Find out more about its unbelievable power in action.

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Want to understand what the correct criteria for column selection should be based on? If so, this tutorial is for you...

Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts?

So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do?

So you're thinking of using gel permeation chromatography (GPC) for your analyses, but before you dive in just what information do you need to know before your first sample injection?

In this technical article you will learn how the signal-to-noise ratio (S/N) is an important variable that may influence the performance of your method.

John Dolan: I often get asked which HPLC system or manufacturer is the best. In days of yore, there was quite a bit of difference in quality and reliability between instruments sold by different manufacturers, but that is no longer true.

This technical article discusses whether high-pressure mixing or low-pressure mixing are better.

Q: I have a validated stability-indicating assay that uses one manufacturer’s C18 column. I know of another column manufacturer where I can get an equivalent C18 column for half the price of the column stated in the method. Will changing columns...