Liquid Chromatography Blog

Developing, Testing and Troubleshooting Chromatography Method Transfer

The transfer of chromatographic methods occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one laboratory to another within the same organization, holding all instrumental and chemical parameters constant. More commonly, however, it is necessary to accommodate changes in available instrumentation.

 
 


We will describe the steps in moving to new instrumentation as well as the role of information rich detectors in confirming a successful transfer that preserves analytical quality and accuracy. The more challenging process of migrating a method to more modern instruments and column chemistry will also be considered.

What will you learn?

  • the key steps in moving to new instrumentation
  • the role of information rich detectors in confirming a successful transfer that preserves analytical quality and accuracy
  • the process of migrating a method to more modern instruments and column chemistries

Thomas WheatThomas E. Wheat earned his Bachelor of Arts in Biology from the College of St. Thomas and his Ph.D. in Cell Biology from the University of Illinois at Urbana-Champaign. He held research positions at Northwestern University before joining Waters Corporation. At Northwestern, his research involved protein purification and structure determination as applied in reproductive immunology. Positions at Waters included Senior Applications Biochemist, Life Sciences Laboratory Manager, and Field Marketing Manager for Mass Spectrometry and Autopurification Systems. He has been involved with the development of several novel techniques for purification and isolation, notably At-column Dilution, as well as tools for protein analysis, including Auto•Blend Plus. He has worked in the practical implementation of orthogonal detection principles in chromatography as well as novel approaches to developing separation methods. He has twice received the Waters President's Club Award for Technical Excellence. He has published and/or presented numerous papers in the fields of analytical biochemistry, capillary electrophoresis, mass spectrometry and preparative chromatography. Frequent presentations have been made worldwide at scientific meetings and other public forums.

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