Liquid Chromatography

This technical article looks at some of the basic calculations used in HPLC, with an emphasis on their practical utility for evaluating separations, developing methods and isolating problems. The first in line is the retention factor, k, often...

In may cases chromatographic processes can be simply described, wrapped in a neat package, and tied up with a pretty bow. This in not the case though with column overload.

There are two aspects of buffer lifetime. One has to do with how long it is effective as a buffer and the second is related to other buffer properties – in particular, microbial growth in the buffer solution.

In this article, we consider whether it is ok to back-flush a column. If a C18 column is used for a routine analysis and the pressure has risen, as in this example, if the column is reversed and unplugged will this be ok or will be the column be...

Looking for new and improved technologies to advance your peptide mapping strategies? Do you want to overcome automation problems with your therapeutic protein workflows? If so, signup for this educational presentation...

DataApex has produced a white paper providing application examples of its Clarity software for analytical and small-scale preparative separation using multivendor analytical and preparative HPLC components.

The Clarity Advanced Chromatography Data Station (CDS) from DataApex provides a universal solution for any commercially available chromatograph. The multi-instrument version offers multi-detector measurement capabilities, data acquisition,...

Thermo Fisher Scientific offers a number of resources to help you learn how charged aerosol detection can be used for both routine and complex analyses and understand more about the versatility of this novel technique.

Do you work in a regulated laboratory? Are you struggling with method transfer? Would you like help with your compliance and data integrity issues? If so, Thermo Fisher Scientific offers a number of resources to help.

There is a growing interest in protein hydrolysates. It has been shown that short bioactive peptides can have higher nutritive value, for example, as antioxidants. In the characterization of protein hydrolysates, it is important to analyse the size...

Screening for mAb aggregation is important in the production of mAb. When performing aggregate analysis on mAbs there is often no issue when the aggregate level is above 1%. At lower levels, however, issues can arise in integration of the aggregate...

Looking for some best practice advice with your titration methods? Working in a regulated laboratory? Would you like a better understanding of compliance and data integrity requirements? If so, this webinar is aimed at you...

The CAMAG^® Derivatizer is an automated spraying device for reagent transfer onto TLC/HPTLC plates setting a new standard of reproducibility and convenience in reagent transfer onto TLC/HPTLC plates by employing a unique “micro droplet” spraying...

Fully automatic high-precision sample application for routine work and laboratories with high sample throughput is achievable using the CAMAG^® Automatic TLC Sampler 4 (ATS 4). Watch the video to see how the productivity in your HPTLC laboratory can...

The CAMAG^® Automatic Developing Chamber 2 (ADC 2) is the key to reproducible results by chromatography under standardized conditions. Watch the video to see how improvements can be made in your HPTLC laboratory...