Liquid Chromatography

Use of Superdex™ 200 Increase size exclusion chromatography columns for screening of mAb aggregates

Screening for mAb aggregation is important in the production of mAb. When performing aggregate analysis on mAbs there is often no issue when the aggregate level is above 1%. At lower levels, however, issues can arise in integration of the aggregate peak. The figure below shows that it is possible to determine ≥ 0.1% aggregate with 4-min run time on Superdex 200 Increase 5/150 GL SEC column. Thus, this column is a good screening tool to evaluate conditions for mAbs.

Figure1Blog5Screening of mAb aggregates on Superdex 200 Increase 5/150 GL at runtimes down to 4 min. Column: Superdex 200 Increase 5/150 GL; Sample: mAb; Sample volume: 5 μL; Flow rate: 0.75 mL/min; System: HPLC system.

Superdex 200 Increase SEC columns are for high-resolution analysis of mAbs and other proteins with Mr~ 10000 to ~ 600000.

Superdex 200 Increase is an agarose-based resin that is alkali resistant and supports cleaning-in-place (CIP) procedures which can increase column lifetime.

Learn more about Superdex 200 Increase SEC columns >>

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