Liquid Chromatography

LC Learning Series: Mastering the art of column choice

Having trouble separating compounds, and wondering what is the single most important parameter to change? Questions over characterizing high molecular weight compounds such as oligonucleotides? Tackle column selection like an expert by registering...

Understanding the role of SPE for clean-up of biological matrices in bioanalysis

Separation Science, in collaboration with Waters, has developed a series of open-access online LC learning courses covering choosing base particles, ligands and hardware for robust chromatographic separations. We are pleased to offer a new full...

Karl Fischer Titration: Three Webinars and a Roundtable

Karl Fischer titration is still the most used, most accurate, and fastest method to determine water content in all kinds of sample matrices.

Fix LC-MS problems with Shimadzu's downloadable troubleshooting guide

LC-MS systems are complex and sensitive instruments, and even minor issues can lead to inaccurate results or instrument downtime, resulting in financial losses and delayed research.

New! Universal HPTLC Mix (UHM) A Novel Concept for HPTLC System Suitability Tests

This article from Issue 14 of the Analytix Reporter discusses theHPTLC system suitability test (SST) which often qualifies only a limited region of the chromatogram. The newly developed Universal HPTLC Mix (UHM) enables TLC users to efficiently and...

Tips & Tricks for Thin-Layer Chromatography

This article from Issue 14 of the Analytix Reporter discusses thin-layer chromatography (TLC), a fast, easy-to-use, cost-efficient, and highly versatile separation technique for qualitative and quantitative analysis. It describes some of the key...

Separation Basics: Fundamental Measurements, More About Resolution and Controlling Selectivity

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we review some basic chromatographic measurements – N, α, k, As, TF, Rs – and how these play into a method development strategy...

HPLC Columns: Packing Characteristics, Alkylsilica Phases and Other Reversed-Phase Options

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the...

Reversed-Phase of Neutral Analytes

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We concentrate on reversed-phase separations in this course, because the majority of HPLC and UHPLC methods use this technique. How does...

Reversed-Phase HPLC of Ionizable Analytes

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. How can we take advantage of pH to improve the separation and what are the pitfalls when we do use pH for this purpose? Why is...

Equivalent & Orthogonal Columns Strategies

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different...

Gradient Separations: How they work

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to...

Quality Issues, Validation and Method Precision

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method...

Quality by Design (QbD) for Better Methods

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and...

Chiral Separations Introduction & Protein Columns

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the...

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