A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different that they make sense to use as a way to change the separation? A free online database containing data on more than 600 reversed-phase columns is examined as a tool to help make column choice decisions.
This course is designed for practical workers in the laboratory who have the responsibility for designing new HPLC or UHPLC methods or transferring methods between laboratories. Experienced workers will get the most out of this class, but because it builds from the fundamentals, anyone with experience in HPLC or UHPLC will gain valuable knowledge.
This course covers:
We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different that they make sense to use as a way to change the separation? A free online database containing data on more than 600 reversed-phase columns is examined as a tool to help make column choice decisions. We show how to use this database to make select two or three orthogonal columns to use in column screening experiments or to use when the original column does not give a satisfactory separation.
- Equivalent Columns
- Orthogonal Columns
- Orthogonal Column Strategy
By attending this online training course you get full access to the 38 video modules and approximately 12.5 hours of instruction. You also get handouts containing copies of all of the approximately 520 PowerPoint slides used in the class. These are arranged for easy note-taking while you view each module and give you a valuable resource for future reference.
This module has a complementary quiz. By correctly answering the quiz questions for all of the modules as part of the course you will be able to download your certificate of completion. Types of questions to expect are:
- What are equivalent columns?
- What are the equivalent columns recommended by the PQRI database?
- What is the best way to find an equivalent column?
- What is an “orthogonal” column?
- What techniques are likely to obtain an orthogonal separation?
- In which cases is an orthogonal column be a good choice to try to improve the separation?
- Why would you choose an orthogonal column?
- What is the best approach when starting out with an unknown sample?
Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently, we offer HPLC training, LC-MS training, GC training and GC-MS training.
