This article from Issue 14 of the Analytix Reporter discusses thin-layer chromatography (TLC), a fast, easy-to-use, cost-efficient, and highly versatile separation technique for qualitative and quantitative analysis. It describes some of the key considerations during the different steps of carrying out the technique.
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Introduction
Thin-layer chromatography (TLC) is a fast, easy-to- use, and highly versatile separation technique for qualitative and quantitative analysis. It is ideal for rapid identification, screening, and reaction monitoring. Its high matrix tolerance and the possibility to separate many samples in parallel makes TLC highly time- & cost-efficient..
Working Principle of TLC
The general thin-layer chromatography process is simple, however it includes a range of steps and some precautions need to be considered (Figure 1) (see full article).
One example is the gas phase in the development chamber, which influences the TLC process. Therefore, it is critical to maintain controlled gas and humidity conditions to obtain reproducible performance and accurate TLC results. We address some of the important aspects, which should be considered at different steps.
Key Considerations in TLC
Learn about the following in detail by reading the full article:
- Storage and handling of TLC plates
- Preparation of TLC plate: pre-rinsing (washing) and activation of the layer
- TLC sample preparation
- Choice of stationary phase in TLC
- How to choose solvent system for TLC?
- Sample application
- Drying plates prior to development
- Pre-conditioning the layer/ humidity control
- Chromatogram/plate development
- Derivatization in TLC
Click below to read the full article.