Liquid Chromatography

Normal Phase & HILIC Separations

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and...

HPLC and UHPLC Advantages

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of...

Advanced HPLC Method Development Using Quality by Design

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Which is usually better, an “adequate” method or an “optimum” method? When beginning method development, is it more effective to start...

Titripac® Sustainable Packaging for Ready-to-Use Certipur® pH Buffers

This article from Issue 13 of the Analytix Reporter shows how the hermetically sealed Titripac® packaging for Certipur® pH buffer solutions prevents contamination and ensures stability and quality of the buffer solutions. Find more about the...

The Carbon Enigma: Material Fundamentals and Retention Properties Of Porous Graphitic Carbon Stationary Phases

As a follow up to the Analytix Reporter Issue 10 article, “Applications Employing New 2.7 μm Porous Graphitic Carbon Particles for U/HPLC”, we explore here, in a shortened review from Issue 13, the more fundamental and theoretical topics concerning...

Synthetic carbon separation media for procss and preparative chromatography

Discover the features, advantages and benefits of synthetic carbon separation media in both liquid and gas purifications and how they bring value to a wide range of LPLC applications for both small and large molecule separations by attending this...

SEC overview of the principles and techniques to obtain reliable protein size-based-separations

Size-Exclusion Chromatography (SEC) is a powerful technique for protein characterization that requires an understanding of the principles to be successful. In this open-access course, we will discuss the history and science of SEC, its strengths and...

How to improve method robustness through control of unwanted interactions

Successful chromatographic separations are as much about preventing the interactions you don’t want, as it is about creating the interactions you need. This open-access course is designed to take you from beginner to advanced in controlling the...

Understanding LC Column Attributes - What's Important for Your Chromatography?

Have you ever wondered how the LC column creates a separation? What attributes are important, what do they do, and how to use them to your advantage? This open-access course is designed to take you from beginner to advanced in understanding column...

What’s New at the Largest Conference in the World Covering All Aspects of Separations and Analyses Carried Out in Liquid-Phase

Join scientists from around the world at the 50th sequel of the longest-running, largest, and most recognized conference in the world covering all aspects of Separations and Analyses carried out in Liquid Phase. HPLC 2022 will take place on June...

Conquering Orbital Ion Trap Limitations

This eBook demonstrates the six coveted advantages you gain with the Agilent 6546 LC/Q-TOF.

Injecting Organic Solvents in Reversed-Phase

A reader wrote me recently asking what would happen if he injected his sample, which came dissolved in hexane, into a reversed-phase mobile phase of methanol-buffer. The first answer is that this is not a good habit, but is it possible? We’ll see.

Column Abuse, I: Does Air Damage the Column?

I recently had an inquiry from a reader of 'HPLC Solutions' that comes up with some regularity in the HPLC troubleshooting classes that I teach: “Will I damage the column by getting air in it?” Let’s consider the possibilities for a moment. One...

Column Abuse 2: Column Storage

A reader asked me if she was in danger of shortening the lifetime of an HPLC column by removing it after each batch of samples and storing it. This also raised the related question about expectations of shorter column lifetimes if the same...

Column Abuse 3: Oops, I Dropped It!

What happens if your favourite HPLC column accidentally rolls off the bench and lands on the concrete floor of the lab? Is it ruined?

Posts by Topic

View more...
Stay ahead and improve your skills!
Receive tailored information on new
separation science methods and applications.
 
Register for free learning now >>