Liquid Chromatography

Separation Science Solutions Series

Recent Posts

Ion suppression in Biological Sample Analysis: You May or May Not See It but It’s There

Interferences exist in every chromatographic analysis whether they are recognized or not. Interferences can be visible in your chromatogram and present themselves as reduced peak area counts, split peaks, shoulders around the peak, or peak...

Understanding Your Sample Preparation Options – Why “Mixing and Matching” Should Be In Your Sample Prep Tool Kit

A successful chromatographic analysis starts with the sample. Sample selection, collection, transportation, preservation, storage, and sampling protocol are all aspects of testing that need to be considered in the context of your analytical...

Food Analysis: What’s Changing and What Are My Sample Prep Options?

While food and beverage imports and exports continue to increase, regulating bodies are taking a greater interest in these activities that can affect public health. Since the early 2000s, worldwide food imports have increased dramatically, as have...

When 'Just Enough' is Not Enough — Part II

In the last column, we discussed ‘just enough’ sample preparation techniques, such as filtration, protein precipitation, extraction partitioning, solid-supported liquid extraction and QuEChERS. Now we will discuss when ‘just enough’ is not enough...

Quantitative Accuracy and Sample Preparation: Pre-Preparation Steps to Improve Results

We’ve covered many of the types of sample preparation options available to make samples ready for instrumental analysis by gas or liquid chromatography and mass spectrometry detection. There are techniques such as QuEChERS, liquid-liquid extraction...

Will QuEChERS Work for Me?

QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) is a sample preparation technique used primarily in the food industry to extract and clean up samples for instrumental analysis. Originally, the QuEChERS approach was developed for multi-residue...

Just Enough Sample Preparation

This technical article shows how sample preparation is an integral part to successful chromatographic analysis and how selecting the right sample preparation technique is dependent upon a number of factors.

The Basics of HILIC

How can we describe the mechanism at the basis of HILIC retention? We will answer this question and give you an insight into the main interactions that govern chromatographic separation in HILIC mode.

Do you HILIC?

Are your analytes eluting close to the void volume of a reversed-phase (RP) column? Do you have problems retaining organic molecules under conventional reversed‑phase liquid chromatography (RPLC) conditions? Have you increased the initial percentage...


The separation power of HPLC combined with the very high sensitivity and selectivity of MS detection is widely used to develop analytical methods that are required to achieve ultra-low detection and quantification limits. Examples of such demanding...

Sample Solvent in HILIC

The sample solvent, also called dissolution solvent or injection solvent, is the solvent used to prepare the solution in which the compound to be analysed by HPLC is dissolved. The choice of the sample solvent is fundamental for a successful...

Column Equilibration in HILIC

It is matter of fact that the samples most chromatographers have to face are rather complex for sample number and compound variety. In these situations, gradient elution is largely preferred for the separation of analytes with different retention...

Column temperature in HILIC

Mostly in HILIC conditions, by increasing column temperature a decrease in the retention time is observed.

HILIC Mobile Phase: pH

This article discusses the pH of the mobile phase that should be selected in order to enhance the hydrophilicity of the analytes.

HILIC Mobile Phase: Salt Content

In this technical article the effect of the salt content in HILIC mobile phases and the preparation is discussed.

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