--- title: Liquid Chromatography Blog | Size Exclusion Chromatography (SEC) description: Size Exclusion Chromatography (SEC) | Webinars, application notes, eBooks, tutorials from the experts by Separation Science for users of liquid chromatography (LC). --- - [Home](https://www.sepscience.com/liquid-chromatography) - [Separation Science](https://www.sepscience.com) - [日本語](https://www.sepscience.com/japan) - [Chromatography Forum](http://www.chromforum.org/) - [Analytical Training Solutions](https://www.analytical-training-solutions.com) - [Contact Us](https://www.sepscience.com/eclipse-business-media/contact-us/) - [Subscribe for free](https://www.sepscience.com/subscribe/) [![Separation Science](https://blog.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) - [Food](https://www.sepscience.com/food) - [Enviro](https://www.sepscience.com/enviro) - [Pharma](https://www.sepscience.com/pharma) - [Bio Clinical](https://www.sepscience.com/bioclinical) - [LC](https://www.sepscience.com/lc) - [GC](https://www.sepscience.com/gc) - [MS](https://www.sepscience.com/ms) - [Methods & Applications](https://www.sepscience.com/methods-and-applications/) - [Webinars & eSeminars](https://www.sepscience.com/webinars-and-eseminars/) - [Videos & Tutorials](https://www.sepscience.com/videos-and-tutorials/) - [Home](https://www.sepscience.com/) - [Get in Touch with Us](https://www.sepscience.com/) - [Home](https://www.sepscience.com/liquid-chromatography) - Liquid Chromatography[Liquid Chromatography](https://blog.sepscience.com/liquidchromatography) - [Liquid Chromatography Blog listing page](https://blog.sepscience.com/liquidchromatography/tag/size-exclusion-chromatography-sec/page/1) # Liquid Chromatography ### [SEC overview of the principles and techniques to obtain reliable protein size-based-separations](https://blog.sepscience.com/liquidchromatography/sec-overview-of-the-principles-and-techniques-to-obtain-reliable-protein-size-based-separations) Size-Exclusion Chromatography (SEC) is a powerful technique for protein characterization that requires an understanding of the principles to be successful. In this open-access course, we will discuss the history and science of SEC, its strengths and... [Read More](https://blog.sepscience.com/liquidchromatography/sec-overview-of-the-principles-and-techniques-to-obtain-reliable-protein-size-based-separations) ### [What’s New at the Largest Conference in the World Covering All Aspects of Separations and Analyses Carried Out in Liquid-Phase](https://blog.sepscience.com/liquidchromatography/whats-new-at-the-largest-conference-in-the-world-covering-all-aspects-of-separations-and-analyses-carried-out-in-liquid-phase) Join scientists from around the world at the 50th sequel of the longest-running, largest, and most recognized conference in the world covering all aspects of Separations and Analyses carried out in Liquid Phase. HPLC 2022 will take place on June... [Read More](https://blog.sepscience.com/liquidchromatography/whats-new-at-the-largest-conference-in-the-world-covering-all-aspects-of-separations-and-analyses-carried-out-in-liquid-phase) ### [PolyAnalysis Forum - New Polymers with Complex Architectures:  Why and how?](https://blog.sepscience.com/liquidchromatography/polyanalysis-forum-new-polymers-with-complex-architectures-why-and-how) Register now for the PolyAnalysis Forum! [Read More](https://blog.sepscience.com/liquidchromatography/polyanalysis-forum-new-polymers-with-complex-architectures-why-and-how) ### [Sample Preparation in GPC/SEC](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sample-preparation-in-gpc/sec) Want to get a better understanding of the various sample preparation procedures available for GPC/SEC analysis, and what to consider when selecting one? If so, this tutorial is for you... [Read More](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sample-preparation-in-gpc/sec) ### [What's New at the Largest Liquid-Phase Separations Conference in the World](https://blog.sepscience.com/liquidchromatography/whats-new-at-the-largest-liquid-phase-separations-conference-in-the-world) Mark your calendar to attend HPLC 2020 on June 20-25, 2020 in beautiful San Diego, CA, USA along the dramatic Pacific coastline. The HPLC conference will celebrate its 50th edition in 2020, and has grown to become the largest and most visible... [Read More](https://blog.sepscience.com/liquidchromatography/whats-new-at-the-largest-liquid-phase-separations-conference-in-the-world) ### [Studying aggregation and degradation over time with SEC](https://blog.sepscience.com/liquidchromatography/studying-aggregation-and-degradation-over-time-with-sec) **Do you suffer from protein aggregation, oligomerization or degradation? If so, analytical SEC is a great diagnostic tool... ** [Read More](https://blog.sepscience.com/liquidchromatography/studying-aggregation-and-degradation-over-time-with-sec) ### [Comparing silica and agarose resins for analysis of proteins with SEC](https://blog.sepscience.com/liquidchromatography/comparing-silica-and-agarose-resins-for-analysis-of-proteins-with-sec) **Silica- and agarose-based resins are the two most used types of size exclusion chromatography (SEC) resins available for protein analysis purposes. But how do they compare and which should you use?** [Read More](https://blog.sepscience.com/liquidchromatography/comparing-silica-and-agarose-resins-for-analysis-of-proteins-with-sec) ### [Shodex size-exclusion chromatography tutorial](https://blog.sepscience.com/liquidchromatography/shodex-size-exclusion-chromatography-tutorial) This video provides a general overview of size-exclusion chromatography.Size-exclusion chromatography is used for analysing high molar mass compounds, such as synthetic polymers, bio-polymers, like proteins and antibodies, polysaccharides and other... [Read More](https://blog.sepscience.com/liquidchromatography/shodex-size-exclusion-chromatography-tutorial) ### [Understanding the benefits of new generation agarose-based SEC resins for biomolecules](https://blog.sepscience.com/liquidchromatography/understanding-the-benefits-of-new-generation-agarose-based-sec-resins-for-biomolecules) **Looking for a solution to biomolecule analysis? Familiar with size exclusion chromatography but need improved analysis? If so, this article outlining new generation agarose-based resins might just be what you need...** [Read More](https://blog.sepscience.com/liquidchromatography/understanding-the-benefits-of-new-generation-agarose-based-sec-resins-for-biomolecules) ### [Leading the Way to Ensure the Separation Sciences Remain a Powerful Analytical Tool](https://blog.sepscience.com/liquidchromatography/leading-the-way-to-ensure-the-separation-sciences-remain-a-powerful-analytical-tool) Mark your calendar to attend HPLC 2018 on July 29-August 2, 2018 in Washington, DC USA. The HPLC conference will celebrate its 47th anniversary in 2018, and has grown to become the largest and most visible international conference series dedicated... [Read More](https://blog.sepscience.com/liquidchromatography/leading-the-way-to-ensure-the-separation-sciences-remain-a-powerful-analytical-tool) ### [Protein analysis with size exclusion chromatography](https://blog.sepscience.com/liquidchromatography/protein-analysis-with-size-exclusion-chromatography) **Looking to improve your protein analysis skills? Interested in how size exclusion chromatography can give you an analytical advantage in this field?** [Read More](https://blog.sepscience.com/liquidchromatography/protein-analysis-with-size-exclusion-chromatography) ### [GPC/SEC Analysis of Polymer Branching](https://blog.sepscience.com/liquidchromatography/gpc/sec-analysis-of-polymer-branching) Malvern has produced an application note describing the GPC (or equivalently SEC) analysis of branching in polyvinylpyrrolidone (PVP). [Read More](https://blog.sepscience.com/liquidchromatography/gpc/sec-analysis-of-polymer-branching) ### [Understanding GPC/SEC - Finding the Right Particle Size and Pore Size](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-finding-the-right-particle-size-and-pore-size) Want to understand what the correct criteria for column selection should be based on? If so, this tutorial is for you... [Read More](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-finding-the-right-particle-size-and-pore-size) ### [Understanding GPC/SEC - Unexpected Peak Shapes](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-unexpected-peak-shapes) Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts? [Read More](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-unexpected-peak-shapes) ### [Understanding GPC/SEC - How to deal with pressure problems](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-how-to-deal-with-pressure-problems) So you're using gel permeation chromatography (GPC) / size exclusion chromatography (SEC) but are experiencing problems with your system pressure. What do you do? [Read More](https://blog.sepscience.com/liquidchromatography/understanding-gpc/sec-how-to-deal-with-pressure-problems) [Next](https://blog.sepscience.com/liquidchromatography/tag/size-exclusion-chromatography-sec/page/2) ### Posts by Topic - [Liquid Chromatography (LC) (173)](https://blog.sepscience.com/liquidchromatography/tag/liquid-chromatography-lc) - [Learning & Training (168)](https://blog.sepscience.com/liquidchromatography/tag/learning-training) - [HPLC (63)](https://blog.sepscience.com/liquidchromatography/tag/hplc) - [Analytical Training Solutions (49)](https://blog.sepscience.com/liquidchromatography/tag/analytical-training-solutions) - [Videos & Tutorials (30)](https://blog.sepscience.com/liquidchromatography/tag/videos-tutorials) - [Methods & Applications (27)](https://blog.sepscience.com/liquidchromatography/tag/methods-applications) - [Method Development (22)](https://blog.sepscience.com/liquidchromatography/tag/method-development) - [Gel Permeation Chromatography (GPC) (21)](https://blog.sepscience.com/liquidchromatography/tag/gel-permeation-chromatography-gpc) - [Size Exclusion Chromatography (SEC) (18)](https://blog.sepscience.com/liquidchromatography/tag/size-exclusion-chromatography-sec) - [LC-MS (17)](https://blog.sepscience.com/liquidchromatography/tag/lc-ms) - [UHPLC (17)](https://blog.sepscience.com/liquidchromatography/tag/uhplc) - [HILIC (16)](https://blog.sepscience.com/liquidchromatography/tag/hilic) - [Webinars & eSeminars (15)](https://blog.sepscience.com/liquidchromatography/tag/webinars-eseminars) - [Analytix Reporter (13)](https://blog.sepscience.com/liquidchromatography/tag/analytix-reporter) - [Events (11)](https://blog.sepscience.com/liquidchromatography/tag/events) - [Karl Fischer Titration (11)](https://blog.sepscience.com/liquidchromatography/tag/karl-fischer-titration) - [Sample Preparation (9)](https://blog.sepscience.com/liquidchromatography/tag/sample-preparation) - [Columns (8)](https://blog.sepscience.com/liquidchromatography/tag/columns) - [Supercritical Fluid Chromatography (SFC) (8)](https://blog.sepscience.com/liquidchromatography/tag/supercritical-fluid-chromatography-sfc) - [TLC / HPTLC (7)](https://blog.sepscience.com/liquidchromatography/tag/tlc-hptlc) - [Food Quality (6)](https://blog.sepscience.com/liquidchromatography/tag/food-quality) - [Protein Analysis (6)](https://blog.sepscience.com/liquidchromatography/tag/protein-analysis) - [Lab Productivity (5)](https://blog.sepscience.com/liquidchromatography/tag/lab-productivity) - [Pharma Drug Discovery (5)](https://blog.sepscience.com/liquidchromatography/tag/pharma-drug-discovery) - [Bioclinical (4)](https://blog.sepscience.com/liquidchromatography/tag/bioclinical) - [Chromatography Software (4)](https://blog.sepscience.com/liquidchromatography/tag/chromatography-software) - [Lab Operations (4)](https://blog.sepscience.com/liquidchromatography/tag/lab-operations) - [Food Flavours & Fragrances (3)](https://blog.sepscience.com/liquidchromatography/tag/food-flavours-fragrances) - [Food Ingredients (3)](https://blog.sepscience.com/liquidchromatography/tag/food-ingredients) - [Food Safety (3)](https://blog.sepscience.com/liquidchromatography/tag/food-safety) - [GPC/SEC (3)](https://blog.sepscience.com/liquidchromatography/tag/gpc-sec) - [ICP-OES (3)](https://blog.sepscience.com/liquidchromatography/tag/icp-oes) - [Ion Chromatography (IC / IC-MS) (3)](https://blog.sepscience.com/liquidchromatography/tag/ion-chromatography-ic-ic-ms) - [LC-MS/MS (3)](https://blog.sepscience.com/liquidchromatography/tag/lc-ms-ms) - [LIMS (3)](https://blog.sepscience.com/liquidchromatography/tag/lims) - [Materials Testing (3)](https://blog.sepscience.com/liquidchromatography/tag/materials-testing) - [News (3)](https://blog.sepscience.com/liquidchromatography/tag/news) - [Preparative-scale Chromatography (3)](https://blog.sepscience.com/liquidchromatography/tag/preparative-scale-chromatography) - [Biopharma (2)](https://blog.sepscience.com/liquidchromatography/tag/biopharma) - [Biopharma Protein & Peptide Analysis (2)](https://blog.sepscience.com/liquidchromatography/tag/biopharma-protein-peptide-analysis) - [Cosmetics (2)](https://blog.sepscience.com/liquidchromatography/tag/cosmetics) - [Data Integrity (2)](https://blog.sepscience.com/liquidchromatography/tag/data-integrity) - [Environmental Water (2)](https://blog.sepscience.com/liquidchromatography/tag/environmental-water) - [Ion Chromatography (2)](https://blog.sepscience.com/liquidchromatography/tag/ion-chromatography) - [LC-UV (2)](https://blog.sepscience.com/liquidchromatography/tag/lc-uv) - [Pharma Quality Assurance (2)](https://blog.sepscience.com/liquidchromatography/tag/pharma-quality-assurance) - [Planar Chromatography (2)](https://blog.sepscience.com/liquidchromatography/tag/planar-chromatography) - [Proteomics (2)](https://blog.sepscience.com/liquidchromatography/tag/proteomics) - [Reversed-Phase Chromatography (2)](https://blog.sepscience.com/liquidchromatography/tag/reversed-phase-chromatography) - [SEC-MALS (2)](https://blog.sepscience.com/liquidchromatography/tag/sec-mals) - [Sampling (2)](https://blog.sepscience.com/liquidchromatography/tag/sampling) - [Solid Phase Microextraction (SPME) (2)](https://blog.sepscience.com/liquidchromatography/tag/solid-phase-microextraction-spme) - [Spectroscopy ICP (2)](https://blog.sepscience.com/liquidchromatography/tag/spectroscopy-icp) - [AI (1)](https://blog.sepscience.com/liquidchromatography/tag/ai) - [CDS (1)](https://blog.sepscience.com/liquidchromatography/tag/cds) - [CRMs (1)](https://blog.sepscience.com/liquidchromatography/tag/crms) - [Cannabis Testing (1)](https://blog.sepscience.com/liquidchromatography/tag/cannabis-testing) - [Charged Aerosol Detection (1)](https://blog.sepscience.com/liquidchromatography/tag/charged-aerosol-detection) - [Convergence Chromatography (UPC) (1)](https://blog.sepscience.com/liquidchromatography/tag/convergence-chromatography-upc) - [Environmental Pesticide Analysis (1)](https://blog.sepscience.com/liquidchromatography/tag/environmental-pesticide-analysis) - [GC-MS (1)](https://blog.sepscience.com/liquidchromatography/tag/gc-ms) - [Gas Chromatography (GC) (1)](https://blog.sepscience.com/liquidchromatography/tag/gas-chromatography-gc) - [ICP-MS (1)](https://blog.sepscience.com/liquidchromatography/tag/icp-ms) - [LPLC (1)](https://blog.sepscience.com/liquidchromatography/tag/lplc) - [Ligand Xchange Chromatography (LEX) (1)](https://blog.sepscience.com/liquidchromatography/tag/ligand-xchange-chromatography-lex) - [PGC (1)](https://blog.sepscience.com/liquidchromatography/tag/pgc) - [Particle technologies (1)](https://blog.sepscience.com/liquidchromatography/tag/particle-technologies) - [Petrochemical (1)](https://blog.sepscience.com/liquidchromatography/tag/petrochemical) - [Pharma Biologics & Biosimilars (1)](https://blog.sepscience.com/liquidchromatography/tag/pharma-biologics-biosimilars) - [Polymer Analysis (1)](https://blog.sepscience.com/liquidchromatography/tag/polymer-analysis) - [Proficiency Testing (1)](https://blog.sepscience.com/liquidchromatography/tag/proficiency-testing) - [Q-TOF (1)](https://blog.sepscience.com/liquidchromatography/tag/q-tof) - [QuEChERS (1)](https://blog.sepscience.com/liquidchromatography/tag/quechers) - [Solid Phase Extraction (SPE) (1)](https://blog.sepscience.com/liquidchromatography/tag/solid-phase-extraction-spe) - [Spectroscopy IR NIR (1)](https://blog.sepscience.com/liquidchromatography/tag/spectroscopy-ir-nir) - [Spectroscopy UV VIS (1)](https://blog.sepscience.com/liquidchromatography/tag/spectroscopy-uv-vis) - [Troubleshooting (1)](https://blog.sepscience.com/liquidchromatography/tag/troubleshooting) - [chromatography data systems (1)](https://blog.sepscience.com/liquidchromatography/tag/chromatography-data-systems) - [consumables (1)](https://blog.sepscience.com/liquidchromatography/tag/consumables) - [eBook (1)](https://blog.sepscience.com/liquidchromatography/tag/ebook) - [intelligent software (1)](https://blog.sepscience.com/liquidchromatography/tag/intelligent-software) - [tutorial (1)](https://blog.sepscience.com/liquidchromatography/tag/tutorial) View more... **Stay ahead and improve your skills!** Receive tailored information on new separation science methods and applications.   [![Register for free learning now >>](https://no-cache.hubspot.com/cta/default/2322077/2a2f6454-cda3-4e5a-97db-ea8546bbb504.png)](https://cta-redirect.hubspot.com/cta/redirect/2322077/2a2f6454-cda3-4e5a-97db-ea8546bbb504)         Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, [info@sepscience.com](mailto:info@sepscience.com)   Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our [Privacy Policy](https://www.sepscience.com/privacy-policy/) or contact [privacy@labxmediagroup.com](mailto:privacy@labxmediagroup.com)  Copyright © 2025. All rights reserved [Contact Us](https://www.sepscience.com/contact-us/) [Advertise with Us](https://www.sepscience.com/advertise-with-us/)