Liquid Chromatography

Having trouble separating compounds, and wondering what is the single most important parameter to change? Questions over characterizing high molecular weight compounds such as oligonucleotides? Tackle column selection like an expert by registering...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we review some basic chromatographic measurements – N, α, k, As, TF, Rs – and how these play into a method development strategy...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We concentrate on reversed-phase separations in this course, because the majority of HPLC and UHPLC methods use this technique. How does...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. How can we take advantage of pH to improve the separation and what are the pitfalls when we do use pH for this purpose? Why is...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of...

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Which is usually better, an “adequate” method or an “optimum” method? When beginning method development, is it more effective to start...

How much sample can you inject into your HPLC system or chosen method? What are the limitations and boundaries? Read more in this edition of 'Tips & Tricks' in Issue 13 of the Analytix Reporter for an overview and advice on injection volumes in HPLC.

Discover the features, advantages and benefits of synthetic carbon separation media in both liquid and gas purifications and how they bring value to a wide range of LPLC applications for both small and large molecule separations by attending this...