Liquid Chromatography

In this technical article you will learn about the two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s)...

In this technical article you will learn how the signal-to-noise ratio (S/N) is an important variable that may influence the performance of your method.

Size exclusion chromatography (SEC) is a key analytical tool for protein characterization, but do you know exactly how it works, and what benefits it offers to the protein scientist?

John Dolan: I often get asked which HPLC system or manufacturer is the best. In days of yore, there was quite a bit of difference in quality and reliability between instruments sold by different manufacturers, but that is no longer true.

This technical article discusses whether high-pressure mixing or low-pressure mixing are better.

Q: I have a validated stability-indicating assay that uses one manufacturer’s C18 column. I know of another column manufacturer where I can get an equivalent C18 column for half the price of the column stated in the method. Will changing columns...

Wyatt Technology, a company specializing in instrumentation for absolute macromolecular and nanoparticle characterization, has announced the launch of its next generation multi-angle light scattering (MALS) detector for SEC/GPC, the miniDAWN^® TREOS^®...

Shimadzu has recently launched its European Innovation Center in Duisburg, Germany. This innovations-oriented 'Think Tank' is a unique approach combining scientific and technological know-how in order to use Shimadzu’s expertise to provide even more...

Successfully transferring a method to a different instrument or laboratory is a challenge faced by most chromatographers. Method transfer might be as simple as migrating an established method to an identical instrument keeping all separation...

This November in Singapore, Separation Science in collaboration with sample preparation expert Ronald Majors, will be providing a one-day training course on sample preparation starteges for good chromatography.