Liquid Chromatography

Equivalent & Orthogonal Columns Strategies

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different...

Gradient Separations: How they work

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to...

Quality Issues, Validation and Method Precision

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method...

Quality by Design (QbD) for Better Methods

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and...

Chiral Separations Introduction & Protein Columns

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the...

Normal Phase & HILIC Separations

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and...

HPLC and UHPLC Advantages

A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of...

Making Life Easier when HPLC and UHPLC Troubleshooting

Do you work with HPLC or UHPLC? "HPLC & UHPLC Troubleshooting: A Performance Qualification Approach” is an intensive online course that teaches you the ins and outs of solving problems that occur with your high-performance liquid chromatography...

Fundamentals of HPLC

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

HPLC Pumps and Degassing

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

HPLC Injectors and Autosamplers

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

HPLC Tubing, Fittings and Hardware

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

HPLC Detectors

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

Selectivity and Efficiency in HPLC

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

Resolution and Asymmetry in HPLC

Are you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and...

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