Liquid Chromatography

In this technical article you will learn how effective trifluoroacetic acid (TFA) in mobile-phase preparation is and how a buffer can be a better choice if a specific pH is required.

This technical article discusses the best way to wash an ion-pairing reagent from a column.

So you're thinking of using gel permeation chromatography (GPC) for your analyses, but before you dive in just what information do you need to know before your first sample injection?

PEEK (poly ether ether ketone) tubing has become a standard item in the operation of many HPLC systems. It is convenient, inexpensive, and easy to identify the internal diameter. However, some care needs to be taken to avoid problems associated with...

In this technical article you will learn about the procedure for purging air from a reversed-phase column.

Q: I know that retention varies according to the ionization of a compound. If the compound is ionized, the retention generally is shorter than if it is not ionized. Does this mean that I should expect two peaks if the compound is near the pKa, where...

This technical article provides details regarding phase dewetting or phase collapse in an HPLC column.

In this technical article you will learn about the two procedures that are used to make sure that an HPLC equipped with a mass spectral detector (MS) is reporting proper mass information and that the system is properly set to detect the analyte(s)...