Liquid Chromatography

Today we often take for granted the quality of the silica in the HPLC column. This may be a reasonable assumption for the high-quality columns produced today, but it is not a guarantee, especially for columns that have been on the market for more...

Earlier this year, I was in London, teaching an HPLC troubleshooting class in conjunction with Separation Science. I love to teach, so the presentation of short courses is one of the highlights of my job. In addition, these classes often serve as...

In this article we’ll consider the last of the initial set of measurements, the column plate-number, N. This is also called the column efficiency, and is calculated as

This technical article considers the factors affecting chromatographic selectivity.

This technical article looks at diagnosing problems with our HPLC separations. 

This technical article looks at several ways to estimate column dead-time.

This technical article looks at some of the basic calculations used in HPLC, with an emphasis on their practical utility for evaluating separations, developing methods and isolating problems. The first in line is the retention factor, k, often...

In may cases chromatographic processes can be simply described, wrapped in a neat package, and tied up with a pretty bow. This in not the case though with column overload.

There are two aspects of buffer lifetime. One has to do with how long it is effective as a buffer and the second is related to other buffer properties – in particular, microbial growth in the buffer solution.

Thermo Fisher Scientific offers a number of resources to help you learn how charged aerosol detection can be used for both routine and complex analyses and understand more about the versatility of this novel technique.

The transfer of chromatographic methods occurs at many stages in the development and production of a product. In its simplest form, the method is moved from one laboratory to another within the same organization, holding all instrumental and...

At the analytica trade exhibition in Munich from 10-13 April, Shimadzu will show the current and new highlights from its entire analytical instrumentation range consisting of chromatography, spectroscopy, spectrometry, TOC, material testing and...

Agilent Technologies has produced a technical overview describing how to combine UV- and mass-based fraction detection with recovery collection for confident, successful sample purification.

Dr Christian Hirsch from Shodex has compiled a comprehensive HPLC column selection guide to help you select the right HPLC column for your application and achieve the best separation out of your HPLC measurements.

...

Mark your calendar to attend HPLC 2018 on July 29-August 2, 2018 in Washington, DC USA. The HPLC conference will celebrate its 47th anniversary in 2018, and has grown to become the largest and most visible international conference series dedicated...