--- title: Liquid Chromatography Blog | Learning & Training description: Learning & Training | Webinars, application notes, eBooks, tutorials from the experts by Separation Science for users of liquid chromatography (LC). --- - [Home](https://www.sepscience.com/liquid-chromatography) - [Separation Science](https://www.sepscience.com) - [日本語](https://www.sepscience.com/japan) - [Chromatography Forum](http://www.chromforum.org/) - [Analytical Training Solutions](https://www.analytical-training-solutions.com) - [Contact Us](https://www.sepscience.com/eclipse-business-media/contact-us/) - [Subscribe for free](https://www.sepscience.com/subscribe/) [![Separation Science](https://blog.sepscience.com/hubfs/Sepscience_Dec2016/images/-resized-strch-SepSci_WebHeader_v3.png)](https://www.sepscience.com/) - [Food](https://www.sepscience.com/food) - [Enviro](https://www.sepscience.com/enviro) - [Pharma](https://www.sepscience.com/pharma) - [Bio Clinical](https://www.sepscience.com/bioclinical) - [LC](https://www.sepscience.com/lc) - [GC](https://www.sepscience.com/gc) - [MS](https://www.sepscience.com/ms) - [Methods & Applications](https://www.sepscience.com/methods-and-applications/) - [Webinars & eSeminars](https://www.sepscience.com/webinars-and-eseminars/) - [Videos & Tutorials](https://www.sepscience.com/videos-and-tutorials/) - [Home](https://www.sepscience.com/) - [Get in Touch with Us](https://www.sepscience.com/) - [Home](https://www.sepscience.com/liquid-chromatography) - Liquid Chromatography[Liquid Chromatography](https://blog.sepscience.com/liquidchromatography) - [Liquid Chromatography Blog listing page](https://blog.sepscience.com/liquidchromatography/tag/learning-training/page/1) # Liquid Chromatography ### [Separation Basics: Fundamental Measurements, More About Resolution and Controlling Selectivity](https://blog.sepscience.com/liquidchromatography/ats/separation-basics-fundamental-measurements-more-about-resolution-and-controlling-selectivity) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we review some basic chromatographic measurements – N, α, k, As, TF, Rs – and how these play into a method development strategy... [Read More](https://blog.sepscience.com/liquidchromatography/ats/separation-basics-fundamental-measurements-more-about-resolution-and-controlling-selectivity) ### [HPLC Columns: Packing Characteristics, Alkylsilica Phases and Other Reversed-Phase Options](https://blog.sepscience.com/liquidchromatography/ats/hplc-columns-packing-characteristics-alkylsilica-phases-and-other-reversed-phase-options) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We examine the processes that influence band spreading and how columns have evolved to their present configuration. The choice of the... [Read More](https://blog.sepscience.com/liquidchromatography/ats/hplc-columns-packing-characteristics-alkylsilica-phases-and-other-reversed-phase-options) ### [Reversed-Phase of Neutral Analytes](https://blog.sepscience.com/liquidchromatography/ats/reversed-phase-of-neutral-analytes) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We concentrate on reversed-phase separations in this course, because the majority of HPLC and UHPLC methods use this technique. How does... [Read More](https://blog.sepscience.com/liquidchromatography/ats/reversed-phase-of-neutral-analytes) ### [Reversed-Phase HPLC of Ionizable Analytes](https://blog.sepscience.com/liquidchromatography/ats/reversed-phase-hplc-of-ionizable-analytes) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. How can we take advantage of pH to improve the separation and what are the pitfalls when we do use pH for this purpose? Why is... [Read More](https://blog.sepscience.com/liquidchromatography/ats/reversed-phase-hplc-of-ionizable-analytes) ### [Equivalent & Orthogonal Columns Strategies](https://blog.sepscience.com/liquidchromatography/ats/equivalent-orthogonal-columns-strategies) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. We return to the column with a look at what makes two columns equivalent and how to determine if two columns are sufficiently different... [Read More](https://blog.sepscience.com/liquidchromatography/ats/equivalent-orthogonal-columns-strategies) ### [Gradient Separations: How they work](https://blog.sepscience.com/liquidchromatography/ats/gradient-separations-how-they-work) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. In this section, we start with an intuitive approach to show how your inherent understanding of isocratic separation can be translated to... [Read More](https://blog.sepscience.com/liquidchromatography/ats/gradient-separations-how-they-work) ### [Quality Issues, Validation and Method Precision](https://blog.sepscience.com/liquidchromatography/ats/quality-issues-validation-and-method-precision) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. This section examines several quality-related issues. We give a brief overview of the validation process and consider when a method... [Read More](https://blog.sepscience.com/liquidchromatography/ats/quality-issues-validation-and-method-precision) ### [Quality by Design (QbD) for Better Methods](https://blog.sepscience.com/liquidchromatography/ats/quality-by-design-qbd-for-better-methods) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Here we look at QbD and how we can use it to help develop more robust methods. We review the variables that affect selectivity and... [Read More](https://blog.sepscience.com/liquidchromatography/ats/quality-by-design-qbd-for-better-methods) ### [Chiral Separations Introduction & Protein Columns](https://blog.sepscience.com/liquidchromatography/ats/chiral-separations-introduction-protein-columns) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Chiral separations are necessary when the sample contains enantiomeric compounds that have “handedness” – that is, they are exactly the... [Read More](https://blog.sepscience.com/liquidchromatography/ats/chiral-separations-introduction-protein-columns) ### [Normal Phase & HILIC Separations](https://blog.sepscience.com/liquidchromatography/ats/normal-phase-hilic-separations) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Normal phase is less used today than reversed-phase, but it can be a powerful tool to separate mixtures containing structural isomers and... [Read More](https://blog.sepscience.com/liquidchromatography/ats/normal-phase-hilic-separations) ### [HPLC and UHPLC Advantages](https://blog.sepscience.com/liquidchromatography/ats/hplc-and-uhplc-advantages) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. UHPLC (ultra-high pressure LC) is rapidly becoming a tool used by many laboratories to speed up the development and application of... [Read More](https://blog.sepscience.com/liquidchromatography/ats/hplc-and-uhplc-advantages) ### [Advanced HPLC Method Development Using Quality by Design](https://blog.sepscience.com/liquidchromatography/ats/advanced-hplc-method-development-using-quality-by-design) A comprehensive course in the systematic development of liquid chromatography separations using QbD principles. Which is usually better, an “adequate” method or an “optimum” method? When beginning method development, is it more effective to start... [Read More](https://blog.sepscience.com/liquidchromatography/ats/advanced-hplc-method-development-using-quality-by-design) ### [A lab-wide approach for training your entire team](https://blog.sepscience.com/liquidchromatography/a-lab-wide-approach-for-training-your-entire-team) A successful lab requires more than just optmized workflows - at its heart it needs a confident, well-trained team of scientists. [Read More](https://blog.sepscience.com/liquidchromatography/a-lab-wide-approach-for-training-your-entire-team) ### [Ion suppression in Biological Sample Analysis: You May or May Not See It but It’s There](https://blog.sepscience.com/liquidchromatography/sample-prep-solutions-7-ion-suppression-in-biological-sample-analysis-you-may-or-may-not-see-it-but-its-there) **Interferences exist in every chromatographic analysis whether they are recognized or not. Interferences can be visible in your chromatogram and present themselves as reduced peak area counts, split peaks, shoulders around the peak, or peak...** [Read More](https://blog.sepscience.com/liquidchromatography/sample-prep-solutions-7-ion-suppression-in-biological-sample-analysis-you-may-or-may-not-see-it-but-its-there) ### [Understanding Your Sample Preparation Options – Why “Mixing and Matching” Should Be In Your Sample Prep Tool Kit](https://blog.sepscience.com/liquidchromatography/sample-prep-solutions-6-understanding-your-sample-preparation-options-why-mixing-and-matching-should-be-in-your-sample-prep-tool-kit) **A successful chromatographic analysis starts with the sample. Sample selection, collection, transportation, preservation, storage, and sampling protocol are all aspects of testing that need to be considered in the context of your analytical...** [Read More](https://blog.sepscience.com/liquidchromatography/sample-prep-solutions-6-understanding-your-sample-preparation-options-why-mixing-and-matching-should-be-in-your-sample-prep-tool-kit) [Next](https://blog.sepscience.com/liquidchromatography/tag/learning-training/page/2) ### Posts by Topic - [Liquid Chromatography (LC) (173)](https://blog.sepscience.com/liquidchromatography/tag/liquid-chromatography-lc) - [Learning & Training (168)](https://blog.sepscience.com/liquidchromatography/tag/learning-training) - [HPLC (63)](https://blog.sepscience.com/liquidchromatography/tag/hplc) - [Analytical Training Solutions (49)](https://blog.sepscience.com/liquidchromatography/tag/analytical-training-solutions) - [Videos & Tutorials (30)](https://blog.sepscience.com/liquidchromatography/tag/videos-tutorials) - [Methods & Applications (27)](https://blog.sepscience.com/liquidchromatography/tag/methods-applications) - 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