Liquid Chromatography

Tosoh Bioscience has produced an application note describing the analysis of asphalt binders using gel permeation chromatography.

Tosoh Bioscience has produced an application describing how to improve reproducibility of MW measurements of polypeptoids using an EcoSEC GPC system.

Tosoh Bioscience has produced an application note describing how to monitor single-chain nanoparticle formation and characterize nanoparticles using multi-pore TSKgel GPC columns.

Shifting retention times within a sample batch can be a real source of headaches in the routine HPLC laboratory. Previously we talked about the kind of retention drift experienced by some HPLC methods for the first few injections. This often is...

Tosoh Bioscience has produced an application note determining the molar mass averages and polydispersity of two polypropylene random copolymer samples via refractive index (RI) detection using the EcoSEC High Temperature GPC System and TSKgel...

How many of you have experienced this? You think you’ve fully equilibrated the HPLC column by running mobile phase through it for 30 min, but for the first 3 or 4 injections, the retention times drift a little, finally settling down after half a...

Gel permeation chromatography (GPC) is a valuable and well established tool for the chemical industry when it comes to the determination of the molecular weight and the molecular weight distribution of polymers.

Tosoh Bioscience has produced an application note demonstrating how GPC was applied to analyse and differentiate the molar mass averages and distributions of four block copolymers intended to be used in a polymer-based therapeutic.

Agilent Technologies has produced a Technical Overview demonstrating the injection principle used in the Agilent 1260 Infinity II SFC Multisampler. In this instrument, the sample volume is drawn under atmospheric pressure conditions, pressurized to...

In this technical article you will learn about which properties are necessary when choosing a compound to use as an internal standard. 

In this technical article you'll learn how an internal standard is used in quantitative analysis.

Agilent Technologies has produced an application note describing a combined approach to analytical method development and method transfer by direct emulation of different target HPLC systems. The proposed workflow presents a general approach to...

Agilent Technologies has produced an application note demonstrating the capability of the Agilent 1260 Infinity Analytical SFC system to perform quantitative analysis of liquid crystal compounds. It demonstrates that fast separation of commercial...

It is easy to take knowledge for granted, especially when you’ve been involved in a field for a long time, as I have with HPLC. One of those areas is about how to protect the column. In the training courses we teach, Tom Jupille and I jokingly...

In an earlier blog, we looked at the processes of sample filtration or centrifugation as a means of reducing or eliminating particulate matter from the injected sample. As I mentioned, I prefer centrifugation, because it is easy, inexpensive and...