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Quality: The Third Side of the Analytical Success Triangle

As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any...

Importance of Limits Option in the NIST Mass Spectral Search Program

The Wiley Registry of Mass Spectral Data has contained a number of “single-peak” spectra such as those shown in Figure 1. Such spectra in an EI database used for the identification of unknowns based on their mass spectra are of no benefit. These...

Field Asymmetric Ion Mobility Spectrometry (FAIMS/DMS) Applications

This technical article is looking at the incorporation of FAIMS/DMS into a conventional LC/MS system to provide an added dimension of ion separation which is orthogonal to both chromatographic and mass spectrometric separation mechanisms. In a...

A Primer on Ion Mobility Spectrometry

Ion mobility spectrometry (IMS), by itself has found a niche in many areas including security screening, environmental monitoring, military applications, and analytical laboratory use. The technique is not mass spectrometry, however it is related...

Choose the Right Interface for LC/MS Success

Many analysts never consider anything beyond the electrospray interface for LC/MS. Electrospray is undeniably a very flexible technique. It works well for macromolecules as well as small molecules, positive and negative ion mode, and is a great...

Use of ECD and ETD Fragmentation Mechanisms for Peptide Sequencing

This technical article describes how two new techniques have arisen which result in more complete fragmentation with better predictability for multiple-charge precursor ions. The techniques are electron capture dissociation (ECD) and electron...

The Importance of Multiple-Charge Ion Precursors in Peptide MS/MS Sequencing

We typically think of a mass spectrum as consisting of a precursor ion at high m/z and a series of increasingly lower m/z product (fragment) ions. These types of mass spectra are shown in training courses and textbooks as didactic examples...

Determination of Intact Protein Molecular Mass from Multiple-Charge Electrospray Mass Spectra

In this technical article the use of multiple-charge ions in protein and peptide analysis, such as the determination of intact protein molecular mass is discussed.

NIST 11: What’s New and What Value Does it Offer? Part 5

Structural Identification of Unknowns from Spectra Obtained Using LC/MS and CAD Techniques and EI Mass Spectral Database.

NIST 11: What’s New and What Value Does it Offer? Part 4

This installment in the series on NIST 11 (National Institute of Standards and Technology) is about the Incremental Name Search , replicate spectra, and the NIST GC Methods, and Retention Index Database.

NIST 11: What’s New and What Value Does it Offer? Part 3

The previous two installments provided information on the NIST (National Institute of Standards and Technology)/EPA/NIH Mass Spectral Database, past and present, and on the use of the NIST MS Search Program in identifying a compound from its mass...

NIST 11: What’s New and What Value Does it Offer? Part 2

NIST 11 (EPA/EPA/NIH Mass Spectral Database of Electron Ionization Data, GC Method/Retention Index Databases, A Database of Spectra Obtained Using MS/MS Techniques, v.2.0g of the NIST MS Search Program, MS Interpreter v.2, and AMDIS) What’s New...

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