As analytical chemists, we spend considerable time thinking about and working with basic scientific principles combined with the technology and products that allow us to solve our analytical problems. However, it is essential for success of any...
Separation Science Mass Spec Solutions
Recent Posts
The Wiley Registry of Mass Spectral Data has contained a number of “single-peak” spectra such as those shown in Figure 1. Such spectra in an EI database used for the identification of unknowns based on their mass spectra are of no benefit. These...
This technical article is looking at the incorporation of FAIMS/DMS into a conventional LC/MS system to provide an added dimension of ion separation which is orthogonal to both chromatographic and mass spectrometric separation mechanisms. In a...
Ion mobility spectrometry (IMS), by itself has found a niche in many areas including security screening, environmental monitoring, military applications, and analytical laboratory use. The technique is not mass spectrometry, however it is related to...
Many analysts never consider anything beyond the electrospray interface for LC/MS. Electrospray is undeniably a very flexible technique. It works well for macromolecules as well as small molecules, positive and negative ion mode, and is a great...
This technical article describes how two new techniques have arisen which result in more complete fragmentation with better predictability for multiple-charge precursor ions. The techniques are electron capture dissociation (ECD) and electron...
We typically think of a mass spectrum as consisting of a precursor ion at high m/z and a series of increasingly lower m/z product (fragment) ions. These types of mass spectra are shown in training courses and textbooks as didactic examples because...
In this technical article the use of multiple-charge ions in protein and peptide analysis, such as the determination of intact protein molecular mass is discussed.
Structural Identification of Unknowns from Spectra Obtained Using LC/MS and CAD Techniques and EI Mass Spectral Database.
This installment in the series on NIST 11 (National Institute of Standards and Technology) is about the Incremental Name Search , replicate spectra, and the NIST GC Methods, and Retention Index Database.
The previous two installments provided information on the NIST (National Institute of Standards and Technology)/EPA/NIH Mass Spectral Database, past and present, and on the use of the NIST MS Search Program in identifying a compound from its mass...
NIST 11 (EPA/EPA/NIH Mass Spectral Database of Electron Ionization Data, GC Method/Retention Index Databases, A Database of Spectra Obtained Using MS/MS Techniques, v.2.0g of the NIST MS Search Program, MS Interpreter v.2, and AMDIS) What’s New and...
The NIST 11 (National Institute of Standards and Technology) Mass Spectral Database, the successor to the NIST 08, is a fully evaluated collection of electron ionization (EI) Mass Spectra, which also includes a growing number of MS/MS Spectra and GC...
In the previous MS Solutions we discussed MS/MS fragmentation of polypeptides, the types of ions formed, and the mechanism of their formation. In this part we will examine a tandem mass spectrum of a simple polypeptide and step through an...
One of the most significant and important applications for mass spectrometry is the sequencing of polypeptides by electrospray LC/MS. An error in the sequence or the substitution of one amino acid with another can completely alter the biological...
Posts by Topic
- Mass Spectrometry (MS) (68)
- Learning & Training (33)
- Webinars & eSeminars (25)
- Spectroscopy ICP (22)
- ICP-MS (13)
- Methods & Applications (12)
- LC-MS (11)
- GC-MS (9)
- Gas Chromatography (GC) (9)
- Spectroscopy Atomic (8)
- Videos & Tutorials (5)
- Biopharma (4)
- ICP-OES (4)
- Ion Chromatography (IC / IC-MS) (4)
- Proteomics (4)
- SIFT-MS (4)
- Chromatography Software (3)
- Environmental Atmospheric (3)
- GC×GC-ToF-MS (3)
- Pharma Drug Discovery (3)
- Two-dimensional Gas Chromatography (GCxGC) (3)
- Columns (2)
- Events (2)
- Food Flavours & Fragrances (2)
- Food Safety (2)
- HPLC (2)
- Method Development (2)
- News (2)
- Spectroscopy IR NIR (2)
- Spectroscopy Raman (2)
- Supercritical Fluid Chromatography (SFC) (2)
- Troubleshooting (2)
- Bioclinical (1)
- Cannabis Testing (1)
- Capillary Electrophoresis (CE) (1)
- Data Integrity (1)
- Electron Activated Dissociation (EAD) (1)
- Environmental (1)
- Food Contaminants (1)
- Food Ingredients (1)
- Forensics (1)
- GC-TOFMS (1)
- Liquid Chromatography (LC) (1)
- MALDI-TOF (1)
- Reversed-Phase Chromatography (1)
- Sample Preparation (1)
- Security Applications (1)
- Spectroscopy NMR (1)
- Spectroscopy UV VIS (1)
- TD-GC-MS (1)
- data independent acquisition (1)
- eBook (1)
- nist (1)
- nist 23 (1)