Mass Spectrometry Blog

In recent years quantitative NMR (qNMR) spectroscopy has become one of the most important tools for content determination of organic substances and quantitative evaluation of impurities. The implementation of qNMR for new application fields,...

In the previous instalment of MS Solutions, I described some of the problems encountered with collisionally-activated dissociation (CAD) of peptides when used in MS/MS sequencing experiments. We noted that the number and type of structurally...

We typically think of a mass spectrum as consisting of a precursor ion at high m/z and a series of increasingly lower m/z product (fragment) ions. These types of mass spectra are shown in training courses and textbooks as didactic examples...

In two previous instalments of MS Solutions, I discussed the sequencing of simple, single-charge peptide ions using MS/MS. In this and another upcoming edition I am going to discuss the use of multiple-charge ions in protein and peptide analysis.

Structural Identification of Unknowns from Spectra Obtained Using LC/MS and CAD Techniques and EI Mass Spectral Database.

This installment in the series on NIST 11 (National Institute of Standards and Technology) is about the Incremental Name Search , replicate spectra, and the NIST GC Methods, and Retention Index Database.

The previous two installments provided information on the NIST (National Institute of Standards and Technology)/EPA/NIH Mass Spectral Database, past and present, and on the use of the NIST MS Search Program in identifying a compound from its mass...

NIST 11 (EPA/EPA/NIH Mass Spectral Database of Electron Ionization Data, GC Method/Retention Index Databases, A Database of Spectra Obtained Using MS/MS Techniques, v.2.0g of the NIST MS Search Program, MS Interpreter v.2, and AMDIS) What’s New...

The NIST 11 (National Institute of Standards and Technology) Mass Spectral Database, the successor to the NIST 08, is a fully evaluated collection of electron ionization (EI) Mass Spectra, which also includes a growing number of MS/MS Spectra and GC...

In the previous MS Solutions we discussed MS/MS fragmentation of polypeptides, the types of ions formed, and the mechanism of their formation. In this part we will examine a tandem mass spectrum of a simple polypeptide and step through an...

One of the most significant and important applications for mass spectrometry is the sequencing of polypeptides by electrospray LC/MS. An error in the sequence or the substitution of one amino acid with another can completely alter the biological...

Mass associated with chemical formulae is different for those working in mass spectrometry as compared to those working in other fields of chemistry and in physics.