This application note describes analysis with the Agilent RapidFire high-throughput MS system using tubing with a smaller inner diameter (id). This way, the necessary sample volume could be minimized.
Minimization of the Required Sample Volume for Agilent RapidFire High-Throughput Mass Spectrometry System
This application note demonstrates a procedure for optimizing a method for a small example molecule, cyclic AMP. The final method has a CV of <3%, greatly improved peak shape, and 20-fold reduced carryover as compared to the generic starting method.
Due to large numbers of samples and the associated analysis time for each, using mass spectrometry (MS) as a primary screening technique can be a long, tedious process. This application note presents a modified Agilent RapidFire high‑throughput MS...