Mass Spectrometry

Peptide Sequencing with Electrospray LC/MS Part 2: Interpretation of a Simple Spectrum

In the previous MS Solutions we discussed MS/MS fragmentation of polypeptides, the types of ions formed, and the mechanism of their formation. In this part we will examine a tandem mass spectrum of a simple polypeptide and step through an...

Peptide Sequencing with Electrospray LC/MS Part 1: Ion Types and Nomenclature

One of the most significant and important applications for mass spectrometry is the sequencing of polypeptides by electrospray LC/MS. An error in the sequence or the substitution of one amino acid with another can completely alter the biological...

Molecular Weight and the Nominal Mass, Monoisotopic Mass and Average Molar Mass

This technical article explains the confusion resulting from molecular weight and the nominal mass, monoisotopic mass and average molar mass.

The Role of Isotope Peak Intensities Obtained Using Mass Spectrometry in Determining an Elemental Composition, Part 2

An illustrative example of the use of isotope peak ratios to determine an elemental composition.

The Role of Isotope Peak Intensities Obtained Using Mass Spectrometry in Determining an Elemental Composition, Part 1

This technical article comprises a discussion of the role of isotope peak intensities obtained using mass spectrometry in determining an elemental composition.

Quantitation in hyphenated chromatographic techniques

Mass spectrometry has long been a valuable quantitative tool used across many industries for numerous applications including food [1], beverage [2], pharma [3] and environmental analysis [4]. Its utility ranges from use as an alternative detector to...

AMDIS – Developing Libraries

by Gary Mallard and O. David Sparkman

For AMDIS to be as effective as possible, it is essential to have high quality target libraries. These can either come from standards taken with the same instrument that the data is analysed on or from the NIST...

AMDIS — Setting Up and Running a Deconvolution and Target Analysis: Part 3

by Gary Mallard and O. David Sparkman

In the first two parts of the discussion of setting up AMDIS the basics of running an analysis with retention index filtering as well as a simple analysis were discussed. In this final part of the set up...

AMDIS – Setting Up and Running a Deconvolution and Target Analysis – Part 2

by Gary Mallard and O. David Sparkman

In the last part of this AMDIS discussion, the setting up and running of a simple analysis was discussed. However, to take full advantage of AMDIS it is far better to make use not only of the mass spectral...

AMDIS: Setting Up and Running a Deconvolution and Target Analysis – Part 1

by Gary Mallard and O. David Sparkman

AMDIS is designed to do analysis for target compounds in a GC/MS data file. To do the analysis you need a completed data file (AMDIS reads most current and many older instrument formats as well as standard...

AMDIS – An Introduction to Extracting High-Quality Spectra from Complex GC/MS Data

by Gary Mallard and O. David Sparkman

The identification of components by GC/MS in complex mixtures has essentially two parts – extracting a spectrum that is due to a single component and then identifying the component, usually through a library...

Introducing a multi-user high-performance MALDI-TOF mass spectrometer

Shimadzu’s MALDI-8020 is the newcomer in their family of MALDI products. This linear MALDI-TOF mass spectrometer combines talents and skills such as outstanding speed, accuracy and performance. It targets researchers developing MALDI-based...

Maximizing value in metals recovery

In a recent interview, Separation Science took time to talk with Adam Currie, Laboratory Manager at Siltech PMR in Melbourne, Australia, to understand how plasma-based techniques such as ICP-OES and MP-AES, and the Agilent OneNeb Series 2 Nebulizer,...

Introducing LECO's Pegasus BT and BT 4D

The LECO Pegasus® BT has been designed to perform qualitative or quantitative analysis of knowns and unknowns. The benchtop instrument combines new, compact time-of-flight mass spectrometer hardware with powerful ChromaTOF brand software, giving...

Determining Terpene Profiles of Cannabis Strains

This presentation from LECO will help you understand the differences between the use of GC and GCxGC with high performance TOFMS and the benefits of using GCxGC for determining terpene profiles of cannabis strains.

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