Gas Chromatography

Dr Matthew Klee

Dr Matthew S. Klee is internationally recognized for contributions to the theory and practice of gas chromatography. His experience in chemical, pharmaceutical, and instrument companies spans over 30 years. During this time, Dr Klee’s work has focused on elucidation and practical demonstration of the many processes involved with GC analysis, with the ultimate goal of improving the ease of use of GC systems, ruggedness of methods and overall quality of results.

Recent Posts

Inlet Liners – Part 3

In this article we continue our liner discussion with a focus on liner volume. One of the most important liner design variables is the internal volume. Larger internal volumes are preferred for general use because they minimize the chance of vapor...

Inlet Liners – Part 2

Here we continue the discussion of inlet liners with a focus on liner outer diameter. It is important to realize that correct liner choice is most critical for:

Inlet Liners – Part 1

Selecting an appropriate inlet liner from the myriad available liner choices is a daunting task, especially when there is little qualified technical information about what differentiates them in terms of actual performance. Here we start discussing...

Activity and Decomposition

Although gas chromatography has many positive attributes as an analytical tool, its incompatibility with labile and active compounds is certainly one of its biggest weaknesses. Here we discuss evidence of sample decomposition and what we might do to...

Split Sample Introduction – Part 2: Measuring Flows, Liner Choice

Continuing our saga of split sample introduction, here we discuss measuring gas flows and liner selection for split injections.

Split Sample Introduction – Part 1: Background and Split Ratio

This technical article introduces the split sample concept and things to take note of for the best analytical results possible.

Saturation of GC Detectors

In this technical article, the causes and effects of saturation and ways to detect and avoid it are discussed.

Hydrogen vs. Helium as Carrier Gas — Hot Discussions

There has been a recent surge in discussions amongst gas chromatographers about switching from helium to hydrogen for carrier gas. Most GC manufacturers, column suppliers, and suppliers of bottled gases and/or gas generators have been feeding the...

Pay Attention to Acquisition Rate and Detector Range

We previously talked about determining limit of detection (LOD) and limit of quantitation (LOQ). I mentioned that it is important to pay attention to the detector acquisition rate and amplifier settings (if they are adjustable) since these can...

How do I Determine Limits of Detection and Quantification?

This technical article addresses the issue of understanding the best way to obtain a limit of detection and quantification. The case discussed involves the quantification of an illicit drug using external calibration.

Why Don’t More People do Fast GC?

In this article I discuss the dichotomy between the often touted benefits of fast GC methods and the proportion of GC methods actually in use. Chromatographers should be aware that one of the two primary advantages of GC over HPLC is its speed (the...

Too Fast GC — What is Too Fast in GC?

Commercialization of technologies to produce faster temperature ramps in GC have been some of the few GC advances in recent decades. This article will take a look at the utility and ultimate areas of practical application for these technologies.

Preventative GC maintenance and performance verification testing

Is the analytical result you are reporting valid? Maybe the ones from yesterday or last week were, but will the ones from today also be? Will the results from your tray of 70 samples have to be thrown out and the analyses be run again? This month we...

Sandwich Injections

In this article we discuss the merits and deficiencies of sandwich injection techniques.

Inlet Activity

In this article we discuss issues related to inlet and liner activity.

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