Liquid Chromatography

Applications for HPLC Analysis

These latest application notes cover a wide range of subjects for HPLC analysis such as separation of water-soluble vitamins, high-throughput bottom-up proteome profiling, mRNA impurity determination, high-throughput analysis of oligonucleotides and characterization of lipid nanoparticle composition. If you're looking to enhance your HPLC analyses, these application notes will be of interest.

 

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the applications demonstrate the use of a UHPLC method development system offering fast method development and include...

Thermo_App1Accelerated method development for the separation of water-soluble vitamins by RP-HPLC with UV detection using an empirical approach to predict separation
This note will demonstrate the application of a software-based method development approach for the advanced separation of all known water-soluble vitamins in reversed-phase mode with ultraviolet detection.

 

Thermo_App3A UHPLC method development system for efficient scouting of chromatographic elution parameters
This note shows the straightforward method development capabilities of the Thermo Scientific™ Vanquish™ UHPLC platform in combination with Thermo Scientific™ Chromeleon™ 7.2 Chromatography Data System (CDS) Software.

 

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Vanquish Neo UHPLC system sets new performance standards for single-shot nanoLCMS bottom-up proteomics
Discover how deep dive proteomics research employing nanoLCMS based analytical systems continues to play a pivotal role in discovery proteomics.

 

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Fast, sensitive, and reproducible nano- and capillary-flow LCMS methods for high−throughput proteome profiling using the Vanquish Neo UHPLC system hyphenated with the Orbitrap Exploris 480 MS
This note demonstrates the performance of the Thermo Scientific™ Vanquish™ Neo UHPLC System coupled to a Thermo Scientific™ Orbitrap Exploris™ 480 Mass Spectrometer for high-throughput bottom−up proteome profiling.

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High-throughput analysis of oligonucleotides using a single quadrupole mass spectrometer for quality control
The goal of this work is to show step-by-step the analysis, deconvolution, and reporting of oligonucleotide synthesis quality control with a single quadrupole mass detector.

 

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Characterization of lipid nanoparticle (LNP) composition using UHPLC-CAD
This note outlines the development of a simple UHPLC method for the characterization of the lipid content of LNP formulations. It demonstrates implementation of the universal CAD detector to quantify large non-chromophoric lipids present in the LNP structure.

 

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Simultaneous reversed-phase and anion-exchange method scouting with a dual system for mRNA impurity determination
This note determines the most suitable conditions for the detection of post-transcriptional impurities in mRNA with a time-effective scouting approach.

 

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Plus you can also download the 'Application Compendium' 

Thermo_App_Compendium_2Successful and stress-free LC method transfers
This compendium offers an all-in-one resource to help tackle the common instrument challenges faced during method transfers to obtain consistent results and sustain regulatory compliance.

 

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