Spectroscopy Solutions

Structural NMR Techniques Contribution to Late Lead Optimization & Preclinical Development

In this on-demand presentation, some examples of applications of different NMR structural techniques with cases from late lead optimization and preclinical development phase will be presented and discussed. Focus will be given also to the combined use of techniques to result in the full physical-chemical profile of the selected drug candidates.

Absolute Quantification of Lipophilic Shellfish Toxin Standards by Quantitative 1H NMR

Learn about accurate quantification of lipophilic toxins in shellfish and why it is important in food safety and often hampered due to lack or limited availability of authentic standards. To solve the problem, we investigated calibration of representative lipophilic shellfish toxin standards, DTX1, OA and YTX, by quantitative NMR (qNMR) using Certified Reference Material (CRM) as internal...

Food Analysis To Check Quality, Safety And Authenticity By Full-Automated 1H-NMR

[On-demand webinar] Full-automated high resolution 1H-NMR spectroscopy offers unique screening capabilities for food quality and safety by combining non-targeted and targeted screening in one analysis (~ 20 minutes from acquisition to report). NMR reproducibility allows statistical investigations e.g. for detection of variety, geographical origin and adulterations, where smallest changes of...

NMR and Molecular Recognition

[On-demand webinar] Molecular recognition by specific targets is at the heart of the life processes. In recent years, it has been shown that the interactions between proteins (lectins, enzymes, antibodies) and carbohydrates mediate a broad range of biological activities, from fertilization, embryogenesis, and tissue maturation, to pathological processes. 

NMR Spectroscopy Applications in Food Analysis

This on-demand webinar lecture by Apostolos Spyros will provide an overview of the different applications of liquid and solid state NMR in food analysis. You will learn about the broad array of analytical problems that can be tackled with NMR spectroscopy, including food authenticity, quality control and adulteration.

Structural NMR Techniques: Contribution to the Drug Discovery & Development

Attend this on-demand webinar by Carla Marchioro and you will learn how structural techniques contribute to drug discovery and development. This will be further explained through application examples.

NMR Solutions # 4: Protein-Detected NMR-Based Methods

NMR-based screening is a powerful experimental technique that can be exploited by employing either ligand- or receptor-based methods [V. Roldós, F.J. Cañada and J. Jiménez-Barbero, Chembiochem., 12, 990-1005 (2011)]. We have already described several protocols operating from the ligand’s perspective (see previous NMR Solutions). Now, we will illustrate the typical scheme employed when analysing...

NMR Solutions #3: The Diffusion Ordered Spectroscopy Technique (DOSY)

The Diffusion Ordered Spectroscopy technique (DOSY) can also be applied to monitor molecular interactions. DOSY is a versatile NMR technique that provides a semiquantitative estimation of the translational diffusion coefficient (D) of a given molecule or mixture of molecules. In fact, the typical representation of DOSY corresponds to a simple 2D spectrum in which one the dimension corresponds to...

NMR Solutions #2: Analysing Protein-Ligand Interactions -  Part 2

In this article, we continue addressing the analysis of ligand-protein interactions by NMR. Now, we will focus on one of the key experiments that monitor the recognition process from the ligand’s viewpoint: 'The Saturation Transfer Difference' method. The employment of this technique permits the existence of interaction and, in favourable cases, the identification of the ligand epitope to be...

NMR Solutions #1: Analysing Protein-Ligand Interactions - Part 1

In the next issues, we will comment on how to set up different experiments to analyse protein-ligand interactions by using NMR spectroscopy. First, we will focus on experiments that monitor the recognition process from the ligand’s viewpoint, starting with the TRNOESY experiment, which allows the conformation of a particular ligand at the receptor’s binding site to be deduced.

NMR Solutions from Bruker

Bruker offers a range of software and hardware that addressses the need for enhanced workflow solutions for NMR spectroscopy research and analysis. These solutions benefit researchers in many disciplines of NMR, while providing productivity enhancements for pharmaceutical users.

NMR Probe for Fluorinated Compounds

JEOL has introduced a probe for NMR analysis of fluorinated compounds prevalent in many new pharmaceutical products.

Protein-Detected NMR-Based Methods – A Powerful Experimental Technique

In this article, Jesús Jiménez Barbero, Scientific Director of CIC bioGUNE, Bizkaia, Spain explains how NMR-based screening is a powerful experimental technique that can be exploited by employing either ligand- or receptor-based methods and illustrates the typical scheme employed when analysing binding from the protein’s point-of-view.