Spectroscopy Solutions Blog

Spectroscopy Solutions Series

Recent Posts

NMR Solutions # 4: Protein-Detected NMR-Based Methods

NMR-based screening is a powerful experimental technique that can be exploited by employing either ligand- or receptor-based methods [V. Roldós, F.J. Cañada and J. Jiménez-Barbero, Chembiochem., 12, 990-1005 (2011)]. We have already described several protocols operating from the ligand’s perspective (see previous NMR Solutions). Now, we will illustrate the typical scheme employed when analysing...

Raman Solutions #4: Validation of Quantitative Raman Methods

This article is the second of a three part series on quantitative prediction using Raman spectroscopy. Method validation is the process of verifying an analytical method is performing as required. Method validation is required in regulated industries and is good scientific practice. United States Pharmacopeia chapters on Near IR spectroscopy and on Raman spectroscopy describe the criteria that...

NMR Solutions #3: The Diffusion Ordered Spectroscopy Technique (DOSY)

The Diffusion Ordered Spectroscopy technique (DOSY) can also be applied to monitor molecular interactions. DOSY is a versatile NMR technique that provides a semiquantitative estimation of the translational diffusion coefficient (D) of a given molecule or mixture of molecules. In fact, the typical representation of DOSY corresponds to a simple 2D spectrum in which one the dimension corresponds to...

Raman Solutions #3: Quantitative Prediction Using Raman Spectroscopy

This article is the first of a three part series on quantitative prediction using Raman spectroscopy. 

NMR Solutions #2: Analysing Protein-Ligand Interactions -  Part 2

In this article, we continue addressing the analysis of ligand-protein interactions by NMR. Now, we will focus on one of the key experiments that monitor the recognition process from the ligand’s viewpoint: 'The Saturation Transfer Difference' method. The employment of this technique permits the existence of interaction and, in favourable cases, the identification of the ligand epitope to be...

Raman Solutions #2: Raman Spectrometer Technology

For the second article of this series of articles on Raman spectroscopy I will review some of the latest in Raman technology. Raman has become one of the most important technologies. The large number of vendors with Raman spectrometers of different sizes is overwhelming.

NMR Solutions #1: Analysing Protein-Ligand Interactions - Part 1

In the next issues, we will comment on how to set up different experiments to analyse protein-ligand interactions by using NMR spectroscopy. First, we will focus on experiments that monitor the recognition process from the ligand’s viewpoint, starting with the TRNOESY experiment, which allows the conformation of a particular ligand at the receptor’s binding site to be deduced.

Raman Solutions #1: The Raman Effect

The Indian physicist, CV Raman, received the Nobel Prize for his discovery of Raman scattering. Raman found that when monochromatic light from a Hg lamp passed through a solid or liquid sample, such as benzene, a small amount of light was scattered in all directions but at a frequency lower (longer wavelength) than the incident light frequency. We note that a group of Russian scientists...

Protein-Detected NMR-Based Methods – A Powerful Experimental Technique

In this article, Jesús Jiménez Barbero, Scientific Director of CIC bioGUNE, Bizkaia, Spain explains how NMR-based screening is a powerful experimental technique that can be exploited by employing either ligand- or receptor-based methods and illustrates the typical scheme employed when analysing binding from the protein’s point-of-view.

How to Validate Quantitative Raman Methods

Frederick H. Long from Spectroscopic Solutions, LLC, New Jersey, USA discusses quantitative prediction using Raman spectroscopy. Method validation is the process of verifying an analytical method is performing as required. It is required in regulated industries and is good scientific practice. United States Pharmacopeia chapters on Near IR spectroscopy and on Raman spectroscopy describe the...

Feed Processing Research with Mid-Infrared Molecular Spectroscopy: Application on Protein in Microwave Irradiation

Peiqiang Yu from the Department of Animal and Poultry Science, College of Agricultural and Bioresources, University of Saskatchewan, Saskatoon, Canada spoke to Spectroscopy Solutions and answered questions on his feed processing research with mid-infrared molecular spectroscopy highlighting an application on protein in microwave irradiation.

  

Subscribe to FREE learning from the experts!