In this article from Issue 11 of the Analytix Reporter, produced by Merck, learn about the Supelco® HPLC columns portfolio and the best practices for connecting the column to any instrument to get maximum performance.
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A Suitability and Installation Review
Since the 1970’s, Supelco® has been a trusted name in high performance liquid chromatography (HPLC) consumables. From a diverse array of columns packed with different stationary phases to a complete portfolio of HPLC accessories, the Supelco® portfolio of HPLC consumables caters to the needs of all analysts. At its most basic level, one major need of the analyst is for the HPLC consumable to be compatible with the instrument. All Supelco™ U/HPLC columns are compatible with instruments from all major vendors and do not require any additional accessories for proper installation.
Supelco® HPLC columns are manufactured with end fittings outfitted with standard 10-32 ports, enabling easy connection to any LC instrument. It is crucial for your HPLC column to be fully in the column oven of your instrument as temperature fluctuations in the laboratory can, in some instances, drastically affect the chromatographic results if a column is only partially in a column oven or if the column oven cannot properly close. Supelco® HPLC columns of all geometries fit comfortably in column ovens of all HPLC, UHPLC, and UPLC® instruments. In addition to the column fitting into a column oven correctly, it is just as important that the column connecting nuts be seated properly in both the inlet and outlet ports of the column. All Supelco columns use Parker fittings, nuts and ferrules, which is the industry standard in HPLC/UHPLC.
In addition to analytical-scale columns, the Supelco® HPLC portfolio also contains capillary and preparative columns for selected stationary phases. Just as with the analytical-scale columns, these capillary and prep columns are compatible with all vendors’ capillary and prep LC systems, respectively.
The Supelco® HPLC portfolio is a comprehensive column and accessories portfolio that is 100% compatible with any HPLC, UHPLC, or UPLC® system. All columns fit within all column ovens from various instrument vendors, and its HPLC accessories can easily be installed onto any instrument without fear of damaging the instrument. Products from the Supelco® HPLC portfolio are a valuable and reliable choice for your U/HPLC or LC-MS workflow.
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