During August 2017, Fipronil was detected in eggs produced from poultry farms in Belgium and The Netherlands. 15 other European countries were also affected prompting the recall of millions of eggs from human consumption. By the end of August 2017, contaminated eggs were also found across the globe including China and Hong Kong. Fipronil, a broad spectrum insecticide which belongs to the phenylpyrazole chemical family, is used in the application of red mite, flea, cockroach and ant control. It is the main active ingredient in many flea prevention pet care products. The World Health Organisation (WHO) has classed Fipronil as a class II moderately hazardous pesticide.
In this application note, a fast and sensitive multi-component single method is described using LC-MS/MS for the detection and quantitation of Fipronil and its associated metabolites, along with Amitraz and its associated metabolites in eggs and other poultry products. Both compounds are used as insecticides on control of fleas and ticks.
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