This application note describes the SFC analysis of choline in a rat cerebrospinal fluid sample by direct injection of the cerebrospinal fluid to the Nexera-UC system.
A CN column provided favourable separation of choline and acetylcholine in SFC-MS/MS analysis. Good linearity of calibration curve, retention time and peak area repeatability were obtained using standard samples. The methods were applied for those compounds in the cerebrospinal fluid sample.
Online SFE-SFC analysis of choline and acetylcholine in a cerebrospinal fluid sample is also shown.
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