Characterization and Comparison of Seven Citrus Fruit Species by Comprehensive LC-MS/MS Analysis of Primary and Secondary Metabolites 

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In this application, the comprehensive analysis of primary and secondary metabolites in seven different species and strains of citrus fruit using an automated method changeover UHPLC-MS/MS system is described. Three different columns and chromatographic conditions were needed to cover the wide range of molecules. The system was configured with a column-switching valve and solvent changer so that the solvent lines and columns were automatically purged and equilibrated to allow the methods to be run seamlessly on the same system.

The results demonstrated that high abundance of carotenoids in flesh was the characteristic feature inherited by species and strains in the taxonomic tree of Citrus unshu (Satsuma/Mikan) and Citrus reticulate (Mandarin orange). Such indication may be useful in making a strategic approach to developing a new strain with better characteristics.

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