Enzymatic cross-linking of caseins with microbial transglutaminase (mTGase) is a prominent example of protein polymerization and shows high potential for techno-functional applications in dairy processing. In many cases, the beneficial effects of the cross-linking were linked to the size of the large casein polymers generated by mTGase, and therefore high-resolution size separation techniques coupled to appropriate detectors are essential for a detailed molecular
characterization.
This application note shows the capabilities of asymmetric flow field flow fractionation (AF4) in combination with differential refractive index measurement plus static and dynamic light scattering (AF4-MALS-DLS) for a gentle separation and investigation of caseins enzymatically cross-linked with mTGase. Using this approach, we have gained essential knowledge regarding the modus operandi of mTGase on size and conformation of caseins.
To download your copy of this application note complete the form opposite.
Registrants for this application note are covered by the Eclipse Business Media Privacy Policy.
Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, info@sepscience.com
Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy or contact privacy@labxmediagroup.com
Copyright © 2023. All rights reserved