In-Well Hydrolysis Using UCT’s Refine™ Ultra-Filtration Technology

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Various biological sample types commonly contain proteins that complicate downstream analysis if not effectively removed. While sample preparation techniques, such as solid phase extraction (SPE), can successfully remove these unwanted matrix components, complex and lengthy extraction procedures are not ideal for high through-put laboratories.

UCT’s Refine™ Ultra-Filtration Technology allows for sample precipitation and filtration to occur simultaneously in the individual column/well. A novel, hydrophobically treated, submicron frit combination facilitates the removal of sample proteins without a complicated extraction. This application note outlines a suggested procedure for in-well hydrolysis of urine samples. Using the Refine™ Ultra-Filtration Technology reduces column backpressure while also reducing analyte suppression, when compared to a standard dilute and shoot protocol.

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