LC-MS based quantification of monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) has been routinely adopted in multiple stages of the biotherapeutic development, serving as an orthogonal technology to the traditional ligand binding assays (LBAs). For small animal PK analysis, the assay sensitivity and throughput are the key factors to evaluate the assay performance. Moreover, scientists have started to work on improving the quantification confidence by generating replicate data from multiple sets of sample preparation.
This application note describes in detail an improved immunoaffinity-micro LC-MS/MS workflow for quantifying trastuzumab emtansine in mouse plasma using the SCIEX 6500+ QTRAP® System with OptiFlow™ Turbo V source and M5 MicroLC System.
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