LC/MS Analysis of Glyphosate and Its Related Compounds

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Shodex advanced HPLC columns has developed a reliable method for the analysis of polar organophosphate-based herbicides.

Organophosphate herbicides, such as glyphosate, have been used as weed killers in agriculture for more than four decades. Due to concerns on their toxicity to humans and the environment, which is discussed in several reviews by international health organizations, many countries have set regulations for the maximum allowed concentration levels for a wide range of crops. The European Food Safety Authority (EFSA) and the U.S. Food and Drug Administration (FDA) organizations, for example, therefore, set maximum allowed concentration ranges between 0.1 and 
310 ppm (equal to mg/L) depending on the crop type. 

In this work a reliable method for high-sensitive direct LC/MS/MS simultaneous analysis of six organophosphate herbicide-related compounds including glyphosate and its metabolite, aminomethylphosphonic acid (AMPA); glufosinate and its metabolites, 3-methylphosphinicopropionic acid (MPPA), ethephon, and fosetyl has been developed. The polymer-based column Shodex™ HILICpak™
VT-50 2D was used for this analysis. Due to the hydrophilic packing material of HILICpak VT-50 2D sufficient retention without derivatization of the analytes or use of ion-pair reagents was obtained. With this method a quantification of the target analytes in 1 ppb (equal to μg/L) concentration levels, which meets the criteria of many regulations, is possible.

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