This application note is aimed at introducing a new and valuable angle to the evaluation of bioprocess by elucidating the state of metabolic pathway in which the target end product is involved. Metabolomic analysis of E.coli extracts have been performed by fast, label-free methods using LC-MS/MS (supplied as the Method Package for Primary Metabolites), which were able to simultaneously monitor the levels of sulfur-containing metabolites, such as cysteine, glutathione, methionine, as well as other metabolites downstream and upstream of the pathway.
The time-course changes in the abundance of metabolites were compared between the same bacterial strain cultured with media supplemented with either thiosulfate or sulfate. The results highlighted how different sectors of the metabolic pathway were affected by respective supplementation of ingredient. This approach is expected to facilitate the designing of bioprocess or manipulation of strain.
To download your copy of this application note complete the form opposite.
Eclipse Business Media Ltd, Regd in England, No. 06513189, Woodview, Bull Lane Industrial Estate, Sudbury, CO10 0FD, United Kingdom, T +44 (0)161 818 7434, info@sepscience.com
Eclipse Business Media Ltd needs the contact information you provide to us to contact you about our products and services. You may unsubscribe from these communications at any time. For information on how to unsubscribe, as well as our privacy practices and commitment to protecting your privacy, check out our Privacy Policy or contact privacy@labxmediagroup.com
Copyright © 2023. All rights reserved