Food Science

Low level quantification of PCDD/Fs in animal feed using GC-MS/MS

In this study from Thermo Fisher Scientific the performance of an enhanced sensitivity triple quadrupole GC-MS/MS was evaluated using both solvent standards and food/foodstuff samples for the analysis of PCDD/Fs to satisfy regulatory requirements.

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Thermo-PCDD-Fs-in-animal-feedIntroduction
Recent developments in gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS) technologies have allowed their use for confirmatory analysis and control of maximum levels (MLs) and action levels (ALs) of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzop-furans (PCDFs), and dioxin-like polychlorinated biphenyls (dl-PCBs) in certain food and feed samples, according to current EU regulations 644/2017 and 771/2017.

Gas chromatography – high-resolution mass spectrometry (GC-HRMS) employs high mass resolution to provide the high selectivity required for the separation of toxic congeners from any interfering matrix components. By contrast, triple quadrupole tandem mass spectrometry (GC-MS/MS) utilizes highly selective, compound-dependent precursor-to-product transitions to obtain total ion chromatograms (TICs) with little or no interference from nonrelated matrix components.

In this study, the performance of the TSQ 9000 triple quadrupole GC-MS/MS system equipped with the new
Advanced Electron Ionization (AEI) source was evaluated for the analysis of PCDD/Fs in solvent standards and real food/feedstuff samples. Suitably set LOQs were employed to show compliance to one-fifth maximum levels and demonstrate the sensitivity, selectivity, and robustness required to operate in a routine environment.

Results and Discussion
Chromatography
The TraceGOLD TG-Dioxin column provided excellent separation for TCDD/F and PeCDD/F congeners and chromatographic resolution compliant with current EU regulations.

Conclusion
The results of this study demonstrate that the TSQ 9000 GC-MS/MS system configured with the AEI source satisfies all of the current EU commission requirements for the detection and confirmation of dioxins in food and feed samples.

Find out more about the analysis of PCDD/Fs in animal feed  >>