This report, from Issue 13 of the Analytix Reporter, offers an overview on the LC-MS elution data of PFAS compounds mentioned in the EPA methods 533, 537.1, and 8327.
PFAS (Per- and poly-fluoroalkyl substances) are persistent, man-made organic compounds, widely found in the environment. Recent awareness has brought attention to the toxicity of these substances. The U.S. Food and Drug Administration (FDA) and the U.S. Environmental Protection Agency (EPA) have initiated actions against PFAS. For determination of PFASs, liquid chromatography–mass spectrometry (LC-MS) is a commonly used technique.
The EPA has developed, validated, and published three methods to support the analysis of 29 PFAS in drinking water, method 533, 537 and 537.1. EPA 8327 covers the analysis of selected per- and polyfluoroalkyl substances (PFAS) in prepared extracts of various matrices (e.g., waters and solids) by liquid chromatography/tandem mass spectrometry (LC-MS/MS) analysis.
The Ascentis® Express PFAS UHPLC column is shown to provide superior and reliable selectivity for a wide range of PFAS compounds.
For full LC-MS conditions and MS transition and retention data download the full article.